Evaluation of the invitro biocompatibility of a new fast-setting ready-to-use root filling and repair material
Evaluation of the invitro biocompatibility of a new fast-setting ready-to-use root filling and repair material
复制标题
新型快速凝固即用型根充修复材料的体外生物相容性评估
DOI：
10.1111/iej.12661
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发表时间：
2017-06-01
影响因子：
5
通讯作者：
Peng, B.
中科院分区：
文献类型：
--
作者：
Lv, F.;Zhu, L.;Peng, B.
AimTo evaluate the invitro biocompatibility of iRoot FS (Innovative BioCeramix Inc., Vancouver, BC, Canada) and to compare its performance with those of iRoot BP Plus (Innovative BioCeramix Inc.) and ProRoot mineral trioxide aggregate (MTA; Dentsply Tulsa Dental, Tulsa, OK, USA).MethodologyMC3T3-E1 cells were cultured for 1, 2 and 3days in various dilutions of iRoot FS, iRoot BP Plus and MTA extracts after 7days of setting to assess cell viability using a cell counting kit-8 (Dojindo, Kumamoto, Japan). The cell apoptosis induced by the set material extracts was evaluated through annexin V-propidium iodide flow cytometry. Changes in the cytoskeletal organization and stress fibres were observed through immunofluorescence by labelling the fibrous actin and nuclei of the cells. Cell attachment was observed under a scanning electron microscope after the MC3T3-E1 cells were cultured on the surface of a material disc set for 1day. The data were analysed using one-way anova.ResultsiRoot FS extracts induced higher cell viability than the control extracts (P
