Toxoplasma gondii: The role of parasite surface and secreted proteins in host cell invasion
Toxoplasma gondii: The role of parasite surface and secreted proteins in host cell invasion
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DOI：
10.1016/0020-7519(95)00103-4
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发表时间：
1996-02-01
影响因子：
4
通讯作者：
Smith, JE
中科院分区：
文献类型：
--
作者：
Grimwood, J;Smith, JE
用体外中和试验研究了弓形虫速殖子5种表面蛋白在宿主细胞入侵中的作用。TG05.54是一种识别主要表面蛋白SAG 1的单抗，它被证明能够持续而显著地阻断牛肾细胞的侵袭，这表明该蛋白在宿主细胞侵袭中发挥了作用，这支持了早期的发现。这种中和作用只在某些抗SAG-1的单抗中观察到，这表明分子内存在一个功能配体。另一种表面蛋白SAG-2也参与了入侵过程。抗SAG2抗体阻止了寄生虫的重新定位，使带状幼虫固定在宿主细胞膜上，从而增加了速殖子的内化。识别其他表面、杆状、致密颗粒和微线体分子的抗体对侵袭没有影响。
The potential role of the 5 surface proteins of Toxoplasma gondii tachyzoites in host cell invasion was investigated using an in vitvo neutralization assay. Supporting earlier findings, TG05.54, a monoclonal antibody recognizing the major surface protein SAG 1, was shown to cause a consistent and significant blockade of invasion into bovine kidney cells, indicating a functional role for this protein in host cell invasion. The neutralizing effect was only seen with certain anti-SAG 1 monoclonal antibodies, suggesting the presence of a functional ligand within the molecule. A second surface protein, SAG 2 was also shown to be involved in the invasion process. Anti-SAG 2 antibodies prevented parasite reorientation, leaving zoites immobilized on the host cell membrane and resulting in increased internalization of tachyzoites. Antibodies recognizing other surface, rhoptry, dense granule and microneme molecules had no effect on invasion.