The red wine antioxidant resveratrol protects isolated rat hearts from ischemia reperfusion injury
The red wine antioxidant resveratrol protects isolated rat hearts from ischemia reperfusion injury
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DOI：
10.1016/s0891-5849(99)00063-5
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发表时间：
1999-07-01
影响因子：
7.4
通讯作者：
Das, DK
中科院分区：
文献类型：
--
作者：
Ray, PS;Maulik, G;Das, DK
据报道，饮用红葡萄酒比饮用其他酒精饮料更有利于预防冠心病。这种有益的效果越来越多地归因于某些抗氧化剂，包括红葡萄酒的多酚部分，如反白藜芦醇。在本研究中，我们研究了白藜芦醇在缺血再灌注（I/R）损伤中的潜在心脏保护作用。在存在或不存在浓度为10 μ M的反白藜芦醇（RVT）的情况下，用Krebs-Henseleit Bicarbonate缓冲液（KHB）灌注稳定后的分离的灌注工作大鼠心脏15分钟，然后使它们经受30分钟的全脑缺血，随后再灌注2小时。在整个再灌注期间的不同时间点监测左心室功能，以评估与基线值相比缺血后恢复的程度。还收集冠状动脉灌注液样品以测定丙二醛（MDA）水平。结果表明，RVT具有明显的心肌保护作用。与对照组（KHB）相比，缺血后心室功能恢复改善，包括压力和主动脉血流。在整个再灌注期间，RVT治疗组的发展压力值显著高于对照组（在再灌注时间点R-15，71.09 +/- 4.88 mmHg对比58.47 +/- 3.88 mmHg，68.87 +/- 5.07 mmHg对比49.74 +/- 2.65 mmHg和51.67 +/- 3.95 mmHg对比30.50 +/- 4.80 mmHg，R-60和R-120）。从R-30开始，与对照组相比，RVT治疗组的主动脉血流量明显更高，在R-90（32.45 +/- 2.19 ml/min vs. 19.83 +/- 1.62 ml/min）和R-120（27.15 +/- 2.27 ml/min vs. 14.10 +/- 1.69 ml/min）时差异最显著。与KHB治疗组相比，RVT治疗组显示出显著减少MDA形成，尤其是在再灌注早期（63.71 +/- 8.19 pM/ml对比130.86 +/- 4.76 pM/ml，63.84 +/- 15.62 pM/ml对比156.99 +/- 18.93 pM/ml，在时间点R-1、R-3、R-5和R-7分别为71.29 +/- 2.80 pM/ml对129.5 +/- 10.30 pM/ml和56.25 +/- 5.79 pM/ml对127.99 +/- 3.50 pM/ml），表明I/R损伤相关的氧化应激减少。与对照组相比，RVT组的颅内压明显减小（10.57 +/- 0.35% vs. 36.27 +/- 5.28%）。体外研究表明，RVT是一种有效的清除剂的过氧自由基的提示可能的机制，涉及的保护能力的RVT。本研究结果表明，白藜芦醇具有心脏保护作用，这可能归因于其清除过氧化氢自由基的活性。(C)1999 Elsevier Science Inc.
The consumption of red wine has been reported to impart a greater benefit in the prevention of coronary heart disease than the consumption of other alcoholic beverages. This beneficial effect is increasingly being attributed to certain antioxidants comprising the polyphenol fraction of red wine such as transresveratrol. In the present study, we investigated the potential cardioprotective effects of resveratrol in the face of ischemia reperfusion (I/R) injury. Isolated perfused working rat hearts after stabilization were perfused with Krebs-Henseleit Bicarbonate buffer (KHB) either in the presence or absence of transresveratrol (RVT) at a concentration of 10 mu M for 15 min prior to subjecting them to 30 min of global ischemia followed by 2 h of reperfusion. Left ventricular functions were monitored at various timepoints throughout the reperfusion period to assess the extent of postischemic recovery in comparison with baseline values. Coronary perfusate samples were also collected to determine malonaldehyde (MDA) levels. The results demonstrated that RVT exhibited significant myocardial protection. This was evidenced by improved recovery of post-ischemic ventricular function including developed pressure and aortic flow as compared to the control group (KHB). Values for developed pressure in the RVT-treated group were significantly higher than those in the control group throughout the reperfusion period (71.09 +/- 4.88 mmHg vs. 58.47 +/- 3.88 mmHg, 68.87 +/- 5.07 mmHg vs. 49.74 +/- 2.65 mmHg and 51.67 +/- 3.95 mmHg vs. 30.50 +/- 4.80 mmHg at reperfusion timepoints R-15, R-60, and R-120, respectively). From R-30 onwards, aortic flow was markedly higher in the RVT treated group as compared with the control group, the differences being most significant at R-90 (32.45 +/- 2.19 ml/min vs. 19.83 +/- 1.62 ml/min) and R-120 (27.15 +/- 2.27 ml/min vs. 14.10 +/- 1.69 ml/min). In contrast to the KHB treated group, the RVT-treated group displayed significant reduction in MDA formation especially in the immediate early reperfusion period (63.71 +/- 8.19 pM/ml vs. 130.86 +/- 4.76 pM/ml, 63.84 +/- 15.62 pM/ml vs. 156.99 +/- 18.93 pM/ml, 71.29 +/- 2.80 pM/ml vs. 129.5 +/- 10.30 pM/ml and 56.25 +/- 5.79 pM/ml vs. 127.99 +/- 3.50 pM/ml at timepoints R-1, R-3, R-5, and R-7, respectively) indicating a reduction in I/R injury related oxidative stress. Infarct size was markedly reduced in the RVT group when compared with the control group (10.57 +/- 0.35% vs. 36.27 +/- 5.28%). In vitro studies revealed RVT to be a potent scavenger of peroxyl radicals suggestive of a probable mechanism involved in the protective ability of RVT. The results of this study indicate that resveratrol possesses cardioprotective effects which may be attributed to its peroxyl radical scavenging activity. (C) 1999 Elsevier Science Inc.