Association of marine viral and bacterial communities with reference black carbon particles under experimental conditions: an analysis with scanning electron, epifluorescence and confocal laser scanning microscopy
Association of marine viral and bacterial communities with reference black carbon particles under experimental conditions: an analysis with scanning electron, epifluorescence and confocal laser scanning microscopy
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DOI：
10.1111/j.1574-6941.2010.00953.x
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发表时间：
2010-11-01
影响因子：
4.2
通讯作者：
Weinbauer, Markus G.
中科院分区：
文献类型：
--
作者：
Cattaneo, Raffaela;Rouviere, Christian;Weinbauer, Markus G.
黑碳(BC)是化石燃料和生物质不完全燃烧的产物，在海洋有机碳库中占有相当大的比例。然而，对BC和海洋微生物之间可能的相互作用知之甚少。在这里，我们报告了使用高浓度标准参考BC材料来调查具有BC颗粒的自然细菌和病毒群落动态的基本原理的实验结果。我们用扫描电子显微镜、荧光显微镜和激光共聚焦扫描显微镜检测了病毒和细菌群落的附着，并用16S rRNA基因变性梯度凝胶电泳(DGGE)检测了细菌群落组成的变化。在24小时的时间历程实验中，BC颗粒显示出很强的吸收病毒和细菌的潜力。在BC处理中，总病毒丰度降低，而总细菌丰度增加。BC颗粒上的病毒和细菌丰度随着颗粒大小的增大而增加，而单位面积上BC相关病毒和细菌的丰度则随着BC颗粒的大小而显著降低。DGGE结果表明，BC具有改变细菌群落结构的潜力，并有利于冰川藻属的亲缘关系。我们的研究表明，BC可以影响海洋生态系统中由细菌和病毒介导的过程。
Black carbon (BC), the product of incomplete combustion of fossil fuels and biomass, constitutes a significant fraction of the marine organic carbon pool. However, little is known about the possible interactions of BC and marine microorganisms. Here, we report the results of experiments using a standard reference BC material in high concentrations to investigate basic principles of the dynamics of natural bacterial and viral communities with BC particles. We assessed the attachment of viral and bacterial communities using scanning electron, epifluorescence and confocal laser scanning microscopy and shifts in bacterial community composition using 16S rRNA gene denaturing gradient gel electrophoresis (DGGE). In 24-h time-course experiments, BC particles showed a strong potential for absorbing viruses and bacteria. Total viral abundance was reduced, whereas total bacterial abundance was stimulated in the BC treatments. Viral and bacterial abundance on BC particles increased with particle size, whereas the abundances of BC-associated viruses and bacteria per square micrometer surface area decreased significantly with BC particle size. DGGE results suggested that BC has the potential to change bacterial community structure and favour phylotypes related to Glaciecola sp. Our study indicates that BC could influence processes mediated by bacteria and viruses in marine ecosystems.