3 MUTANTS THAT EXTEND BOTH MEAN AND MAXIMUM LIFE-SPAN OF THE NEMATODE, CAENORHABDITIS-ELEGANS, DEFINE THE AGE-1 GENE
3 MUTANTS THAT EXTEND BOTH MEAN AND MAXIMUM LIFE-SPAN OF THE NEMATODE, CAENORHABDITIS-ELEGANS, DEFINE THE AGE-1 GENE
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DOI：
10.1093/geronj/43.4.b102
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发表时间：
1988-07-01
期刊：
影响因子：
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通讯作者：
JOHNSON, TE
中科院分区：
文献类型：
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作者：
FRIEDMAN, DB;JOHNSON, TE
对线虫线虫中的长寿突变体进行了研究，以确定导致寿命延长的突变是否是等位基因。所研究的四个突变株中有三个(MK31、MK542、MK546)含有显著延长寿命的隐性突变；MK542和MK546始终未能补充AGE-1的长寿表型，因此MK31是等位基因，尽管MK31的肝脏比野生型长，但在某些情况下未能补充AGE-1，但在其他情况下不是。所有三个长寿菌株都降低了两性人的自我生育能力，也无法补充AGE-1突变的这种假定的多效性效应。这三个菌株中的每一个都在UNC-31 IV处包含一个独立的突变。由于这些突变是在同一次突变搜索(Klass，1983)中使用不保证独立性的方案分离出来的，因此不能假设这些突变是独立分离的。
Long-lived mutants in the nematode Caenorhabditis elegans have been studied to determine if the mutations responsible for extended life were allelic. Three of four mutant strains studied (MK31, MK542, MK546) contain recessive mutations that significantly lengthen life; MK542 and MK546 consistently fail to complement the long life phenotype of age-1 and are therefore allelic MK31, although longer liver than wild type, is equivocal, in some cases failing to complement age-1 but not in others. All three long-lived strains have reduced hermaphrodite self-fertility and also fail to complement for this presumed pleiotropic effect of the age-1 mutation. Each of these three strains also contains an independent mutation at unc-31 IV. Since the mutants were isolated in the same mutant hunt (Klass, 1983) using protocols that did not guarantee independence, the mutations cannot be assumed to be independently isolated.