Investigation of extracellular polymeric substances (EPS) properties of P. aeruginosa and B. subtillis and their role in bacterial adhesion
Investigation of extracellular polymeric substances (EPS) properties of P. aeruginosa and B. subtillis and their role in bacterial adhesion
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DOI：
10.1016/j.colsurfb.2016.06.039
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发表时间：
2016-10-01
影响因子：
5.8
通讯作者：
Ting, Yen-Peng
中科院分区：
文献类型：
--
作者：
Harimawan, Ardiyan;Ting, Yen-Peng
生物膜中的胞外聚合物（EPS）基质具有重要的功能，如对抗菌剂的扩散屏障，使得生物膜细胞更难以完全消除。因此，生物膜细胞表现出增强的弹性，不像嗜酸性细胞，并且更难以完全消除。为了获得细菌粘附到表面的全面了解，需要在生长和生物膜形成过程中产生的EPS的组成和构象特性的知识，因为它们的粘附和构象特性在分子水平上仍然知之甚少。目前的研究提供了进一步的见解，以确定组成和构象特性的EPS产生的嗜酸性和生物膜细胞的B。枯草杆菌和铜绿假单胞菌。各种光谱分析表明，两种不同物种产生的EPS在化学上是不同的。B的EPS中含有较多的蛋白质类化合物。枯草芽孢杆菌的EPS的特征在于更大的碳水化合物组分，而铜绿假单胞菌的EPS的特征在于更大的碳水化合物组分。然而，多糖和/或蛋白质组分的相对比例随细菌的生长模式而变化。然后使用AFM通过使用单分子力光谱（SMFS）来探测由细菌产生的EPS的粘附性质。两种细菌的比较表明，多糖的存在下，促进EPS的粘附强度，而蛋白质有较小的粘附效果。相同细菌菌株的两种生长模式的比较还表明，更大的EPS产生和增强的细胞粘附与生物膜生长相关。(C)2016爱思唯尔B. V.保留所有权利。
Extracellular polymeric substances (EPS) matrix in biofilm poses important functions such as a diffusion barrier to antimicrobial agents so that biofilm cells are more difficult to completely eliminate. Therefore, biofilm cells exhibit enhanced resilience unlike planktonic cells, and are more difficult to completely eliminate. In order to obtain a comprehensive understanding of bacterial adhesion to surfaces, knowledge of the composition and conformational properties of EPS produced during growth and biofilm formation is required, since their adhesive and conformational properties remain poorly understood at molecular level. Present study has provided further insights into identifying compositional and conformational properties of EPS produced by planktonic and biofilm cells of B. subtills and P. aeruginosa. Various spectroscopy analyses showed that EPS produced by the two different species were chemically dissimilar. More proteinaceous compounds were present in EPS from B. subtilis, while EPS from P. aeruginosa were characterized by greater carbohydrate components. However, relative proportions of polysaccharides and/or proteins constituents varied with the growth mode of the bacteria. AFM was then used to probe the adhesive nature of EPS produced by the bacteria by using Single Molecule Force Spectroscopy (SMFS). Comparison of the two bacterial species indicated that the presence of polysaccharides promoted the adhesion strength of the EPS while proteins had lesser adherence effects. Comparison of the two growth modes for the same bacterial strain also indicated that greater EPS production and enhanced cellular adhesion are associated with biofilm growth. (C) 2016 Elsevier B.V. All rights reserved.