Role of Staphylococcus aureus surface adhesins in orthopaedic device infections: Are results model-dependent?
Role of Staphylococcus aureus surface adhesins in orthopaedic device infections: Are results model-dependent?
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DOI：
10.1099/00222615-46-1-75
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发表时间：
1997-01-01
影响因子：
3
通讯作者：
Klima, M
中科院分区：
文献类型：
--
作者：
Darouiche, RO;Landon, GC;Klima, M
假体材料的细菌定植可能导致临床感染或植入物失效，或两者兼而有之，通常需要取出器械。金黄色葡萄球菌与生物假体材料的粘附由属于微生物细胞表面蛋白MSCRAMM（识别粘附基质分子的微生物表面组分）家族的粘附素介导。本研究的目的是比较S.金黄色葡萄球菌纽曼，具有所有三种纤维蛋白原，纤连蛋白和胶原结合MSCRAMM（MSCRAMM阳性菌株）与缺乏所有三种类型的MSCRAMM（MSCRAMM阴性菌株）的突变菌株在骨科器械相关感染的兔模型。在每只动物的膝关节上钻孔后，一组10只兔子接种MSCRAMM阳性菌株，另一组10只兔子接种MSCRAMM阴性菌株。然后将不锈钢螺钉放入钻孔中。两周后，处死兔子，采集血清样本、骨组织和植入物进行细菌学和组织病理学评价。两组间感染率无显著差异。描述S.金黄色葡萄球菌表面粘附素引起骨科器械相关感染可能具有模型依赖性。
Bacterial colonisation of prosthetic material can lead to clinical infection or implant failure, or both, often requiring removal of the device. Adherence of Staphylococcus aureus to bioprosthetic materials is mediated by adhesins belonging to the MSCRAMM (microbial surface components recognising adhesive matrix molecules) family of microbial cell surface proteins. The objective of this study was to compare the virulence of a mutant strain of S. aureus Newman that possesses all three fibrinogen-, fibronectin- and collagen-binding MSCRAMMs (MSCRAMM-positive strain) with that of a mutant strain that lacks all three types of MSCRAMMs (MSCRAMM-negative strain) in a rabbit model of orthopaedic device-related infection. After a hole was drilled into the knee joint of each animal, a group of 10 rabbits was inoculated with the MSCRAMM-positive strain and another group of 10 rabbits received the MSCRAMM-negative strain. A stainless steel screw was then placed into the drilled hole. Two weeks later, the rabbits were killed and serum samples, bone tissue and implants were harvested for bacteriological and histopathological evaluation. No significant difference in infection rates was demonstrated between the two groups. The ability to delineate the role of S. aureus surface adhesins in causing orthopaedic device-related infection could be model-dependent.