Naive human T cells develop into Th1 or Th0 effectors and exhibit cytotoxicity early after stimulation with Leishmania-infected macrophages.
Naive human T cells develop into Th1 or Th0 effectors and exhibit cytotoxicity early after stimulation with Leishmania-infected macrophages.
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DOI：
10.1086/515284
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发表时间：
1998-05
期刊：
影响因子：
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通讯作者：
D. Russo;P. Chakrabarti;J. Burns
中科院分区：
文献类型：
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作者：
D. Russo;P. Chakrabarti;J. Burns
人类疾病的研究表明，自然获得性免疫是利什曼原虫感染的主要结果。通常，在无症状或自愈性感染期间激活的保护性免疫机制在发生疾病的患者中可能是最小的。为了探索早期免疫应答，开发了人利什曼原虫感染的体外模型，其中用利什曼原虫感染的巨噬细胞致敏幼稚T细胞。通过这些T细胞系对利什曼原虫特异性细胞因子产生的分析显示，大多数个体在感染后早期产生了Th 1或Th 0应答。Th 1应答者感染的巨噬细胞产生白细胞介素-12。Th 0应答者产生很少或不产生内源性白细胞介素-12，可通过在引发过程中加入白细胞介素-12转化为Th 1表型。最后，感染致敏的T细胞特异性裂解利什曼原虫感染的巨噬细胞。因此，该体外模型系统可用于描绘针对感染后早期诱导的利什曼原虫的保护性人类免疫应答。
Studies of human disease suggest that naturally acquired immunity is the predominant outcome of Leishmania infection. Normally protective immune mechanisms activated during asymptomatic or self-healing infections may be minimal in patients who develop disease. To explore early immune responses, an in vitro model of human Leishmania infection was developed in which naive T cells were sensitized with Leishmania-infected macrophages. An analysis of Leishmania-specific cytokine production by these T cell lines revealed that most individuals developed Th1 or Th0 responses early after infection. Infected macrophages from Th1 responders produced interleukin-12. Th0 responders who produced little or no endogenous interleukin-12 could be converted to the Th1 phenotype by addition of interleukin-12 during priming. Finally, infection-sensitized T cells specifically lysed Leishmania-infected macrophages. Thus, this in vitro model system can be used to delineate protective human immune responses against Leishmania induced early after infection.