Cadherin-mediated cell interaction regulates germ cell determination in mice
Cadherin-mediated cell interaction regulates germ cell determination in mice
复制标题
DOI：
10.1242/dev.00870
复制
发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Matsui, Y
中科院分区：
文献类型：
--
作者：
Okamura, D;Kimura, T;Matsui, Y
在早期胚胎中，生殖细胞谱系与体细胞谱系分离。小鼠的生殖细胞决定不受母系遗传的种质的调节，而是在原肠胚形成期间在胚胎内启动。然而，小鼠生殖细胞特化的机制仍然未知。我们位于原始生殖细胞（PGCs）的前体在早期胚胎，并在这里显示，这些前体之间的细胞-细胞相互作用是需要生殖细胞规格。我们发现，钙依赖性细胞粘附分子，E-钙粘蛋白的表达，被限制在胚胎外中胚层，包含PGC前体的近端区域，并阻断E-钙粘蛋白的功能与抗体抑制PGC的形成在体外。这些结果表明，E-钙粘蛋白介导的细胞间相互作用的细胞含有PGC前体是必不可少的指导这些细胞的生殖细胞的命运。
The germ cell lineage segregates from the somatic cell lineages in early embryos. Germ cell determination in mice is not regulated by maternally inherited germplasm, but is initiated within the embryo during gastrulation. However, the mechanisms of germ cell specification in mice remain unknown. We located precursors to primordial germ cells (PGCs) within early embryos, and show here that cell-cell interaction among these precursors is required for germ cell specification. We found that the expression of a calcium-dependent cell adhesion molecule, E-cadherin, is restricted to the proximal region of extra-embryonic mesoderm that contains PGC precursors, and that blocking the functions of E-cadherin with an antibody inhibits PGC formation in vitro. These results showed that E-cadherin-mediated cell-cell interaction among cells containing PGC precursors is essential to directing such cells to the germ cell fate.