Thyrotropin releasing hormone but not histidyl-proline diketopiperazine is depleted from rat spinal cord following 5,7-dihydroxytryptamine treatment.
Thyrotropin releasing hormone but not histidyl-proline diketopiperazine is depleted from rat spinal cord following 5,7-dihydroxytryptamine treatment.
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5,7-二羟色胺治疗后，大鼠脊髓中的促甲状腺激素释放激素而非组氨酰脯氨酸二酮哌嗪被耗尽。
DOI：
10.1016/0006-8993(85)91395-2
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Jackson,IM
中科院分区：
文献类型：
--
作者：
Lechan,RM;Jackson,IM
组氨酸脯氨酸二酮哌嗪（His-Pro DKP）被认为是促甲状腺激素释放激素（TRH）的代谢物。脊髓TRH起源于脑干5-羟色胺（5- ht）神经元，我们将5- ht神经毒素5,7-二羟色胺（5,7- dht）注射到7只大鼠侧脑室，5周后观察脊髓TRH和hs - pro DKP的水平。与生理盐水处理的对照组相比，5,7- dht处理的动物显示整个脊髓的TRH明显减少，特别是在腰骶区，几乎90%的TRH消失（0.28±0.02vs2.46±0.01ng/mg蛋白；P< 0.0001）。与此相反，民主党在各地区没有明显的变化。由于5,7- dht通过破坏髓质的核周TRH来降低脊髓TRH，我们得出结论，脊髓hs - pro DKP不是来自与TRH相同的神经元。
Histidyl-proline diketopiperazine (His-Pro DKP) has been proposed as a metabolite of thyrotropin releasing hormone (TRH). Since spinal cord TRH arises from serotoninergic (5-HT) neurons in the brainstem, a 5-HT neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), was injected into the lateral ventricle of 7 rats, and the levels of TRH and His-Pro DKP in the spinal cord were studied 5 weeks later. In comparison to the saline treated controls, 5,7-DHT treated animals showed marked depletion of TRH throughout the spinal cord, especially in the lumbosacral area where almost 90% disappeared, (0.28 ± 0.02vs2.46 ± 0.01ng/mg protein;P< 0.0001). In contrast, His-Pro DKP showed no significant change in any region. Since 5,7-DHT lowers spinal cord TRH by destroying TRH perikarya in the medulla, we conclude that spinal cord His-Pro DKP is not derived from the same neurons as TRH.