The 3a protein of severe acute respiratory syndrome-associated coronavirus induces apoptosis in Vero E6 cells
The 3a protein of severe acute respiratory syndrome-associated coronavirus induces apoptosis in Vero E6 cells
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DOI：
10.1099/vir.0.80813-0
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发表时间：
2005-07-01
影响因子：
3.8
通讯作者：
Tsui, SKW
中科院分区：
文献类型：
--
作者：
Law, PTW;Wong, CH;Tsui, SKW
中国爆发了严重急性呼吸系统综合症（SARS）， 2002年11月中旬出现了第一例病例。发现该病的病原是一种以前未知的冠状病毒，即sars相关冠状病毒（SARS-CoV）。sars - cov感染的详细病理和宿主对病毒感染的反应尚不清楚。3a基因编码一种非结构病毒蛋白，预计是一种跨膜蛋白。本研究发现，3a蛋白在SARS患者的肺和肠组织中表达，在转染3a的猴肾Vero E6细胞中，该蛋白定位于内质网。体外染色质凝集和DNA断裂实验表明，3a蛋白可能引发细胞凋亡。这些数据表明，过表达单个sars冠状病毒蛋白可诱导体外细胞凋亡。
An outbreak of severe acute respiratory syndrome (SARS) occurred in China and the first case emerged in mid-November 2002. The aetiological agent of this disease was found to be a previously unknown coronavirus, SARS-associated coronavirus (SARS-CoV). The detailed pathology of SAIRS-CoV infection and the host response to the viral infection are still not known. The 3a gene encodes a non-structural viral protein, which is predicted to be a transmembrane protein. In this study, it was shown that the 3a protein was expressed in the lungs and intestinal tissues of SARS patients and that the protein localized to the endoplasmic reticulum in 3a-transfected monkey kidney Vero E6 cells. In vitro experiments of chromatin condensation and DNA fragmentation suggested that the 3a protein may trigger apoptosis. These data showed that overexpression of a single SARS-CoV protein can induce apoptosis in vitro.