Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis
Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis
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通过基于凝胶的质谱分析策略直接消化活细胞
DOI：
10.1039/c6cc08316a
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发表时间：
2017
影响因子：
4.9
通讯作者：
Lu Haojie
中科院分区：
文献类型：
--
作者：
Xiong Yun;Zhang Ying;Yao Jun;Yan Guoquan;Lu Haojie
建立了一种新的蛋白质组学样品制备方法，将活细胞吸附到真空干燥的聚丙烯酰胺凝胶中，直接消化成多肽，用于后续的LC-MS/MS分析。因此，跳过了常规消化方法中涉及的细胞裂解和蛋白质提取两个步骤。
A novel sample preparation method was established for proteomic analysis, during which living cells were absorbed into vacuum-dried polyacrylamide gel and directly digested into peptides for subsequent LC-MS/MS assays. As a consequence, both of the steps for cell lysis and protein extraction involved in a conventional digestion method were skipped.