Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro
Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro
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DOI：
10.1046/j.1460-9568.1999.00756.x
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发表时间：
1999-10-01
影响因子：
3.4
通讯作者：
Cohen, J
中科院分区：
文献类型：
--
作者：
Gavazzi, I;Kumar, RDC;Cohen, J
在背根神经节中的成人初级感觉神经元的不同亚群表达不同营养因子的受体，并且因此潜在地响应于不同的营养信号。我们已经比较了神经营养因子神经生长因子（NGF），脑源性神经营养因子（BDNF）和NT-3，和胶质细胞系源性神经营养因子（GDNF）的神经突起生长在分离培养的感觉神经元从年轻的成年大鼠腰神经节的影响，并试图建立这些营养因子的亚组特异性的影响。我们分析了三个参数的神经突生长（过程轴承神经元的百分比，最长的神经突的长度和总的神经突长度），这可能与特定类型的轴突生长在体内，因此可能会有不同的反应营养因子的存在。我们的研究结果表明，突起轴承神经元的百分比和总轴突长度的营养因子的影响，而最长的轴突的长度是营养因子无关。在体外实验中，发现只有NGF和GDNF能显著提高突起神经元的比例。GDNF对小型IB 4（-）神经元比NGF更有效，已知这些神经元在出生后发育早期会产生GDNF反应。NGF和GDNF在较小程度上增强了培养中神经元产生的神经突的总长度。BDNF对生长有抑制作用，BDNF和NT-3都能部分阻断NGF对特定神经元亚群的促生长作用。
Different subpopulations of adult primary sensory neurons in the dorsal root ganglia express receptors for different trophic factors, and are therefore potentially responsive to distinct trophic signals. We have compared the effect of the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and NT-3, and of glial cell line-derived neurotrophic factor (GDNF) on neurite outgrowth in dissociated cultures of sensory neurons from the lumbar ganglia of young adult rats, and attempted to establish subset-specific effects of these trophic factors. We analysed three parameters of neurite growth (percentage of process-bearing neurons, length of longest neurite and total neurite length), which may correlate with particular types of axon growth in vivo, and may therefore respond differently to trophic factor presence. Our results showed that percentage of process-bearing neurons and total neurite length were influenced by trophic factors, whilst the length of the longest neurite was trophic factor independent. Only NGF and GDNF were found to enhance significantly the proportion of process-bearing neurons in vitro. GDNF was more effective than NGF on small, IB4(-) neurons, which are known to develop GDNF responsiveness early in postnatal development. NGF, and to a much lesser extent GDNF, enhanced the total length of the neurites produced by neurons in culture. BDNF exerted an inhibitory effect on growth, and both BDNF and NT-3 could partially block some of the growth-promoting effects of NGF on specific neuronal subpopulations.