EFFECT OF POLY(ETHYLENE GLYCOL) ON PHOSPHOLIPID HYDRATION AND POLARITY OF THE EXTERNAL PHASE
EFFECT OF POLY(ETHYLENE GLYCOL) ON PHOSPHOLIPID HYDRATION AND POLARITY OF THE EXTERNAL PHASE
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DOI：
10.1016/0005-2736(83)90444-3
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
GAWRISCH, K
中科院分区：
文献类型：
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作者：
ARNOLD, K;PRATSCH, L;GAWRISCH, K
用2 H-NMR研究了磷脂酰胆碱（PC）/水分散体在聚乙二醇（PEG）存在下的水化性质。的四极分裂和它们的温度依赖性对应于PC/水分散体在低水含量的测量。结合水被PEG部分提取，但约5个水分子的内部水化壳层中的水的结合性质没有改变。一些磷脂/水分散体的能力进行相变的非层状结构脱水后进行了讨论。二棕榈酰磷脂酰胆碱（DPPC）和卵磷脂不形成非层状结构上添加纯化的PEG，如31 P-NMR所示。PEG降低了水相的极性，并且改变了膜和外相之间的疏水分子的分配。这是证明使用准分子荧光的芘在鬼悬浮液。加入PEG后的极性和水合作用的变化可能有助于在膜接触和融合条件下观察到的膜表面的改变。
The hydration properties of phosphatidylcholine (PC)/water dispersions on the addition of poly(ethylene glycol) [PEG] were studied by 2H-NMR. The quadrupole splittings and their temperature dependences correspond to measurements of PC/water dispersions at low water content. The bound water is partly extracted by PEG but the binding properties of the water in the inner hydration shell of about 5 water molecules are not changed. The ability of some phospholipid/water dispersions to undergo phase transitions to nonlamellar structures upon dehydration is discussed. Dipalmitoylphosphatidylcholine (DPPC) and egg phosphatidylcholine do not form nonlamellar structures on addition of purified PEG, as was demonstrated by 31P-NMR. PEG decreases the polarity of the aqueous phase and the partition of hydrophobic molecules between the membrane and the external phase is changed. This was demonstrated using the excimer fluorescence of pyrene in a ghost suspension. The changes in polarity and hydration on the addition of PEG may contribute to the alterations in the membrane surface observed under conditions of membrane contact and fusion.