Phenylalanine 4-Hydroxylase Contributes to Endophytic Bacterium Pseudomonas fluorescens' Melatonin Biosynthesis.
Phenylalanine 4-Hydroxylase Contributes to Endophytic Bacterium Pseudomonas fluorescens' Melatonin Biosynthesis.
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苯丙氨酸 4-羟化酶有助于内生细菌荧光假单胞菌的褪黑素生物合成
DOI：
10.3389/fgene.2021.746392
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发表时间：
2021
影响因子：
3.7
通讯作者：
Pang H
中科院分区：
文献类型：
--
作者：
Jiao J;Xia Y;Zhang Y;Wu X;Liu C;Feng J;Zheng X;Song S;Bai T;Song C;Wang M;Pang H
褪黑激素在植物中既作为抗氧化剂又作为生长调节物质。荧光假单胞菌内生细菌已被证明产生褪黑激素，并通过增加内源褪黑激素来增加植物对非生物胁迫的抗性。然而，在细菌中，基因仍然不知道是褪黑激素相关的。本文报道了细菌苯丙氨酸4-羟化酶（PAH）可能参与荧光假单胞菌5-羟色氨酸（5-HTP）的生物合成，并进一步影响其褪黑素的合成。纯化的荧光假单胞菌PAH蛋白不仅具有苯丙氨酸的羟基化作用，而且在体外还具有L-色氨酸（L-Trp）羟化酶活性，可将L-Trp转化为5-HTP。然而，细菌PAH表现出较低的活性和亲和力的l-Trp比l-苯丙氨酸。值得注意的是，荧光假单胞菌的PAH缺失通过引起5-HTP水平的显著下降来阻断褪黑激素的产生，从而降低了对非生物胁迫的抗性。总体而言，这项研究揭示了细菌PAH在控制5-HTP和褪黑激素的生物合成在细菌中可能发挥的作用，并扩大了目前的知识褪黑激素生产的微生物。
Melatonin acts both as an antioxidant and as a growth regulatory substance in plants. Pseudomonas fluorescens endophytic bacterium has been shown to produce melatonin and increase plant resistance to abiotic stressors through increasing endogenous melatonin. However, in bacteria, genes are still not known to be melatonin-related. Here, we reported that the bacterial phenylalanine 4-hydroxylase (PAH) may be involved in the 5-hydroxytryptophan (5-HTP) biosynthesis and further influenced the subsequent production of melatonin in P. fluorescens. The purified PAH protein of P. fluorescens not only hydroxylated phenylalanine but also exhibited l-tryptophan (l-Trp) hydroxylase activity by converting l-Trp to 5-HTP in vitro. However, bacterial PAH displayed lower activity and affinity for l-Trp than l-phenylalanine. Notably, the PAH deletion of P. fluorescens blocked melatonin production by causing a significant decline in 5-HTP levels and thus decreased the resistance to abiotic stress. Overall, this study revealed a possible role for bacterial PAH in controlling 5-HTP and melatonin biosynthesis in bacteria, and expanded the current knowledge of melatonin production in microorganisms.
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影响因子：
10.3
作者：
Arnao, M. B.;Hernandez-Ruiz, J.
通讯作者：
Hernandez-Ruiz, J.
DOI：
10.1006/bbrc.2002.6719
发表时间：
2002-04-05
影响因子：
3.1
作者：
Daubner, SC;Moran, GR;Fitzpatrick, PF
通讯作者：
Fitzpatrick, PF
影响因子：
2.8
作者：
HARTMANN, A;SINGH, M;KLINGMULLER, W
通讯作者：
KLINGMULLER, W
影响因子：
10.3
作者：
Byeon, Yeong;Lee, Hye-Jung;Back, Kyoungwhan
通讯作者：
Back, Kyoungwhan
影响因子：
10.3
作者：
Byeon, Yeong;Park, Sangkyu;Back, Kyoungwhan
通讯作者：
Back, Kyoungwhan