Rapid adaptive remote focusing microscope for sensing of volumetric neural activity.
Rapid adaptive remote focusing microscope for sensing of volumetric neural activity.
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DOI：
10.1364/boe.8.004369
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发表时间：
2017-10-01
影响因子：
3.4
通讯作者：
Booth MJ
中科院分区：
文献类型：
--
作者：
Žurauskas M;Barnstedt O;Frade-Rodriguez M;Waddell S;Booth MJ
以细胞分辨率记录生物体大脑中的神经活动的能力对于确定指导行为的神经回路机制非常重要。在这里，我们提出了一种自适应双光子显微镜，即使在样本运动的情况下，也能优化提取完整果蝇大脑中体积上的神经信号。通过降低空间分辨率，在保持高分辨率、高数值孔径显微镜的光收集效率的同时，高速体积成像成为可能。这使得能够同时记录在活果蝇体内特定体积的蘑菇体Kenyon细胞体中气味诱发的钙瞬变。
The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact Drosophila brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly.