In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes.
In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes.
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使用“蓝色”染料对哺乳动物皮层进行体内电压敏感染料成像。
DOI：
10.1101/pdb.prot089359
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发表时间：
2015
影响因子：
--
通讯作者：
Wu,J-Y
中科院分区：
文献类型：
--
作者：
Baker,Bradley;Gao,Xin;Wolff,BrianS;Jin,Lei;Cohen,LawrenceB;Bleau,ChunX;Wu,J-Y
膜电位的光学记录允许从神经系统的许多不同位置同时进行测量。这对于神经系统的研究很重要，因为神经系统的同时活动可以发生在区域、细胞和亚细胞水平上。 Amiram Grinvald 团队开发的新型“蓝色”染料是哺乳动物皮层体内电压敏感染料成像的一大进步。蓝色染料被红光 (630 nm) 激发，与血红蛋白的光吸收 (510–590 nm) 不重叠。这实际上消除了心脏搏动伪影。
Optical recording of membrane potential allows simultaneous measurements to be taken from many different locations in the nervous system. This is important in studies of the nervous system in which simultaneous activity can occur at the regional, cellular, and subcellular levels. New “blue” dyes, developed by Amiram Grinvald’s group, are a great advance for in vivo voltage-sensitive dye imaging of mammalian cortex. The blue dyes are excited by red light (630 nm) that does not overlap with light absorption of hemoglobin (510–590 nm). This virtually eliminates the heart pulsation artifact.