Syne-1, a dystrophin- and klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction
Syne-1, a dystrophin- and klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction
复制标题
DOI：
10.1074/jbc.m004775200
复制
发表时间：
2000-10-13
影响因子：
4.8
通讯作者：
Sanes, JR
中科院分区：
文献类型：
--
作者：
Apel, ED;Lewis, RM;Sanes, JR
我们描述了一种新的蛋白质，Syne-1，这是与骨骼肌，心脏和平滑肌细胞的核膜。Syne-1包含多个血影蛋白重复序列，类似于在肌营养不良蛋白和utrophin中发现的那些，以及与Klarsicht的羧基末端同源的结构域，Klarsicht是一种与细胞核相关的蛋白质，并且是果蝇中细胞核迁移的一个子集所需的。在成年骨骼肌纤维中，Syne-1的水平在位于神经肌肉接头处的突触后膜下方的核中最高。这些细胞核是转录特化的，在同一细胞质中比突触外细胞核更高水平地表达突触成分的基因。Syne-1是第一个被发现与突触核选择性结合的蛋白质。Syne-1在出生后集中在突触核中。它在去神经支配或变性/再生后保持突触富集，并且也以高水平存在于营养不良肌管的中央核中。Syne-1的位置和结构表明，它可能参与肌核在肌管中的迁移和/或它们在突触后装置的锚定。最后，我们确定了一个同源基因，syne-2，这是在一个重叠，但不同的模式表达。
We describe a novel protein, Syne-1, that is associated with nuclear envelopes in skeletal, cardiac, and smooth muscle cells. Syne-1 contains multiple spectrin repeats similar to those found in dystrophin and utrophin, as well as a domain homologous to the carboxyl-terminal of Klarsicht, a protein associated with nuclei and required for a subset of nuclear migrations in Drosophila. In adult skeletal muscle fibers, levels of Syne-1 are highest in the nuclei that lie beneath the postsynaptic membrane at: the neuromuscular junction. These nuclei are transcriptionally specialized, expressing genes for synaptic components at higher levels than extrasynaptic nuclei in the same cytoplasm. Syne-1 is the first protein found to be selectively associated with synaptic nuclei. Syne-1 becomes concentrated in synaptic nuclei postnatally. It remains synaptically enriched following denervation or degeneration/regeneration, and is also present at high levels in the central nuclei of dystrophic myotubes. The location and structure of Syne-1 suggest that it may participate in the migration of myonuclei in myotubes and/or their anchoring at the postsynaptic apparatus. Finally, we identify a homologous gene, syne-2, that is expressed in an overlapping but distinct pattern.