RNAi mediated silencing of lipoxygenase gene to maintain rice grain quality and viability during storage
RNAi mediated silencing of lipoxygenase gene to maintain rice grain quality and viability during storage
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DOI：
10.1007/s11240-014-0476-6
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Datta, Swapan K.
中科院分区：
文献类型：
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作者：
Gayen, Dipak;Ali, Nusrat;Datta, Swapan K.
脂氧合酶（LOX）是一种常见的酶，它催化种子脂质过氧化，从而导致种子品质恶化，降低种子活力。种子贮藏过程中，由于LOX活性的增强，不饱和脂肪酸发生过氧化反应，直接导致种子活力下降，籽粒营养品质恶化。本研究利用RNAi技术降低LOX活性，以克服水稻种子贮藏过程中存在的这些问题。为了提高种子贮藏稳定性，我们利用LOX基因的一个功能片段，在组成型启动子（CaMV35S）和淀粉蛋白特异性启动子（Oleosin-18）的控制下，分别下调LOX基因的活性。为了解RNAi-LOX种子和非转基因对照种子的贮藏稳定性，在45℃、85%相对湿度条件下加速老化14 d。我们的研究表明，降低LOX活性可以减少种子在贮藏条件下的品质劣化。此外，GC-MS分析显示，非转基因水稻种子在贮藏过程中脂肪酸含量的下降幅度高于转基因水稻种子。此外，LOX活性降低的转基因水稻种子贮藏后的种子萌发效率高于非转基因水稻种子。研究结果将直接影响优质稻米的营养稳定性。
Lipoxygenase (LOX) is a common enzyme which catalyzes lipid peroxidation of seeds and consequently enhances seed quality deterioration and decreases seed viability. During seed storage, peroxidation of unsaturated fatty acids occur due to enhancement of LOX activity which directly leads to reduction in seed vigour and deterioration of grain nutritional quality. This study was undertaken to overcome these problem during rice seed storage by attenuating LOX activity using RNAi technology. To improve seed storage stability, we down regulated LOX gene activity by using a functional fragment of the LOX gene under the control of both constitutive (CaMV35S) and aleurone-specific (Oleosin-18) promoter separately. To understand the storage stability, RNAi-LOX seeds and non-transgenic control seeds were subjected to accelerated aging at 45 A degrees C and 85 % relative humidity for 14 days. Our studies demonstrate that down regulation of LOX activity reduces the seed quality deterioration under storage condition. In addition GC-MS analysis revealed that reduction of fatty acid level in non-transgenic seeds during storage was higher when compared with that of transgenic rice seeds. Furthermore, the transgenic rice seeds with reduced LOX activity exhibited enhanced seed germination efficiency after storage than that of non-transgenic rice seeds. This study will have direct impact on nutritional stability of quality rice grains.