Claudin 6 expression is useful to distinguish myxofibrosarcomas from other myxoid soft tissue tumors
Claudin 6 expression is useful to distinguish myxofibrosarcomas from other myxoid soft tissue tumors
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DOI：
10.1016/j.prp.2016.12.001
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Oda, Yoshinao
中科院分区：
文献类型：
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作者：
Bekki, Hirofumi;Yamamoto, Hidetaka;Oda, Yoshinao
粘液纤维肉瘤（MFS）的特点是丰富的粘液样间质，广泛的细胞学异常和频繁的局部复发。部分软组织肿瘤伴黏液样间质，组织学上与MFS相似，但生物学行为不同。在这里，我们试图确定一个有用的诊断标记MFS。在我们分析了来自基因表达综合数据库的基因表达数据集之后，我们专注于claudin 6（CLDN 6）。通过免疫组化在61例MFS和其他（良性）粘液样软组织肿瘤（28例粘液瘤样本，12例结节性筋膜炎样本），18例低度纤维粘液样肉瘤，30例粘液样脂肪肉瘤，29例皮肤外粘液样软骨肉瘤和27例具有粘液样特征的去分化脂肪肉瘤样本中评估CLDN 6的状态。同时分析CLDN6的表达与MFS临床病理特征的关系.免疫组化结果显示，在大约100例正常人中，CLDN 6表达量较高。65%的MFS，而良性软组织肿瘤没有显示CLDN 6的高表达。CLDN6在MFS中的表达明显高于其他肿瘤组织.在MFS中，CLDN 6的高表达与高FNCLCC分级和高AJCC分期相关。CLDN 6可作为MFS与良性黏液样肿瘤鉴别诊断及预测其侵袭性生物学行为的参考指标。(C)2016 Elsevier GmbH. All rights reserved.
Myxofibrosarcoma (MFS) is characterized by abundant myxoid stroma, a wide spectrum of cytological atypia, and frequent local recurrence. Some soft tissue tumors with myxoid stroma can histologically mimic MFS, but have different biological behaviors. Here we sought to identify a useful diagnostic marker for MFS. After our analysis of the gene expression dataset from the Gene Expression Omnibus database, we focused on claudin 6 (CLDN 6). The status of CLDN 6 was assessed by immunohistochemistry in 61 samples of MFS and other (benign) myxoid soft tissue tumors (28 myxoma samples, 12 nodular fasciitis samples), 18 low-grade fibromyxoid sarcoma, 30 myxoid liposarcoma, 29 extraskeletal myxoid chondrosarcoma and 27 dedifferentiated liposarcoma with myxoid feature samples. The correlation between the expression of CLDN 6 and clinicopathological findings in MFS was also investigated. Immunohistochemically, high expression of CLDN 6 was observed in approx. 65% of the MFSs, whereas the benign soft tissue tumors did not show a high expression of CLDN 6. The expression of CLDN 6 in the MFS was significantly higher than those of other tumor specimens. Among the MFSs, the high expression of CLDN 6 was correlated with high FNCLCC grades and high AJCC stages. CLDN 6 may be useful for the differential diagnosis from benign myxoid tumor and for predicting the aggressive biological behavior of MFS. (C) 2016 Elsevier GmbH. All rights reserved.