Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus)
Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus)
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麝香鼠 (Ondatra zibethicus) 气味腺中 SF-1、StAR 和 P450scc 的季节性表达
DOI：
10.1016/j.jsbmb.2020.105766
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发表时间：
2020-11-01
影响因子：
4.1
通讯作者：
Weng, Qiang
中科院分区：
文献类型：
--
作者：
Xie, Wenqian;Tang, Zeqi;Weng, Qiang
甾体生成发生在哺乳动物的特定细胞和组织中，始于胆固醇转化为孕烯醇酮的转移和细胞内加工。本研究研究了甾体生成因子1 （SF-1）、甾体生成急性调节蛋白（StAR）和细胞色素P450胆固醇侧链裂解酶（P450scc）在麝鼠繁殖期和非繁殖期嗅觉腺中的基因和蛋白表达水平。StAR和P450scc的免疫组化定位于腺细胞和上皮细胞，而SF-1仅在繁殖期和非繁殖期在腺细胞中表达。嗅觉腺中SF-1、StAR和P450scc的基因和蛋白表达量在繁殖期明显高于非繁殖期。micrornas （miRNAs）与转录组的相互作用结果表明，miR-762和miR-4454可能是关键生物学过程中编码（Nr5a1、Star和Cyp11a1）的基因。综上所述，这些结果表明，麝鼠的气味腺可能具有重新合成类固醇激素的能力，类固醇激素可能会影响麝鼠在繁殖期和非繁殖期的气味腺功能。
The steroidogenesis occurs in specific cells and tissues in the mammals which begins with the transfer and intracellular processing of cholesterol converted to pregnenolone. This study investigated the gene and protein expression levels of steroidogenic factor 1 (SF-1), steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) in the scent glands of the muskrats during the breeding and non-breeding seasons. The immunohistochemical localizations of StAR and P450scc were identified in the glandular cells and epithelial cells while SF-1 was only expressed in glandular cells during the breeding and non breeding seasons. The gene and protein expression levels of SF-1, StAR and P450scc in the scent glands were remarkedly higher in the breeding season than those of the non-breeding season. The interaction of micro RNAs (miRNAs) and transcriptome results showed that miR-762 and miR-4454 might be the genes encoding (Nr5a1, Star and Cyp11a1) in key biological processes. Taken together, these results suggested that the scent glands of the muskrats potentially owned ability to synthesize steroid hormones de novo , and the steroid hormones might affect the scent glandular functions of the muskrats during the breeding and non-breeding seasons.