Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus
Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus
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DOI：
10.1016/s1050-4648(03)00113-x
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发表时间：
2004-03-01
影响因子：
4.7
通讯作者：
Chen, JC
中科院分区：
文献类型：
--
作者：
Liu, CH;Chen, JC
氨氮浓度在0～20 mg·l(-1)范围内的TSB培养基对溶藻弧菌的生长没有影响。用溶藻弧菌攻击白虾凡纳滨对虾（蜕皮间期7-12克），溶藻弧菌已在含有不同浓度氨-N（0、1、5、10和20 mg l(-1)）的TSB培养基中孵育24 It。在含有0、1、5、10和20 mg l(-1)氨氮的TSB培养基中孵育的虾在攻击120小时后，累积死亡率没有显着差异。虾用预先在 TSB 培养基中孵育 24 小时的溶藻弧菌进行攻击，然后放入氨氮浓度为 0.01 mg l(-1)（对照）、1.10、5.24、11.10 和 21.60 mg l(-1) 的水中。 5.24、11. 10和21.60 mg l(-1)溶液中的虾在48-168 h后的死亡率显着高于对照溶液(0.01 mgl(-1))。暴露于对照、1.10、5.24、11.10和21.60 mg l(-1)氨氮7天的虾检测THC（总血细胞计数）、颗粒细胞、透明细胞、酚氧化酶活性、超氧阴离子释放、超氧化物歧化酶（SOD）活性、吞噬活性和对溶藻弧菌的清除效率。不同氨氮浓度下，虾的THC、透明细胞和颗粒细胞没有观察到显着差异。然而，当虾暴露于 5.24 mg l(-1) 氨氮或 7 天后更高浓度时，酚氧化酶活性下降。当氨氮浓度为21.60 mg·l(-1)时,超氧阴离子释放显着增加,而SOD活性显着降低。虾暴露于11.21和21.22 mg l(-1)氨氮7天后，对溶藻弧菌的吞噬活性和清除效率显着下降。因此，有人认为水中的氨会导致免疫反应抑制，并增加南美白对虾因溶藻弧菌感染而导致的死亡率。 (C) 2003 Elsevier Ltd. 保留所有权利。
Growth of Vibrio alginolyticus was not affected by TSB medium containing ammonia-N concentration in the range of 0-20 mg l(-1). White shrimp Litopenaeus vannamei (7-12 g in the intermolt stage) were challenged with V alginolyticus, which had been incubated for 24 It in the TSB medium containing different concentrations of ammonia-N (0, 1, 5. 10 and 20 mg l(-1)). There was no significant difference in cumulative mortality for shrimp incubated in the TSB medium containing 0, 1, 5, 10 and 20 mg l(-1) ammonia-N after 120 h of challenge. The shrimps were challenged with V alginolyticus previously incubated in the TSB medium for 24 h, then placed in water containing concentrations of ammonia-N at 0.01 mg l(-1) (control), 1.10, 5.24, 11.10 and 21.60 mg l(-1). Mortality of shrimp in 5.24, 11. 10 and 21.60 mg l(-1) was significantly higher than those in the control solution (0.01 mgl(-1)) after 48-168 h. Shrimps which had been exposed to control, 1.10, 5.24, 11.10 and 21.60 mg l(-1) ammonia-N for 7 days were examined for THC (total haemocyte count), granular cells, hyaline cells, phenoloxidase activity, release of superoxide anion, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency to V alginolyticus. No significant difference in THC, hyaline cells and granular cells were observed among shrimps at different ammonia-N concentrations. Phenoloxidase activity however, decreased when the shrimps were exposed to 5.24 mg l(-1) ammonia-N and greater after 7 days. The release of superoxide anion increased significantly, whereas SOD activity decreased significantly at 21.60 mg l(-1) ammonia-N. With shrimps exposed to 11.21 and 21.22 mg l(-1) ammonia-N for 7 days, phagocytic activity and clearance efficiency to V alginolyticus significantly decreased. It is therefore suggested that ammonia in water caused a depression in the immune response and an increase in mortality of L. vannamei from the V. alginolyticus infection. (C) 2003 Elsevier Ltd. All rights reserved.