Preparation, characterization and improvement in intestinal function of polysaccharide fractions from okra
Preparation, characterization and improvement in intestinal function of polysaccharide fractions from okra
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DOI：
10.1016/j.jff.2018.09.035
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发表时间：
2018-11-01
影响因子：
5.6
通讯作者：
Zhang, Huawei
中科院分区：
文献类型：
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作者：
Gao, Han;Zhang, Wencheng;Zhang, Huawei
本研究旨在探讨分子量相近的黄秋葵多糖对肠道生态的影响。经提取、醇沉、脱蛋白、纯化后得到水提多糖和碱提多糖。高效液相色谱分析结果表明，AEP中含有甘露糖、鼠李糖、半乳糖醛酸和半乳糖，而WEP中含有半乳糖、鼠李糖、果糖、阿拉伯糖、葡萄糖、半乳糖醛酸和甘露糖。肠道菌群的组成受每日底物的影响。同时，氨浓度和胃肠通过时间下降，总短链脂肪酸（SCFA）水平，盲肠含水量增加。这些发现表明，黄秋葵多糖对肠道菌群的影响是通过有害化合物的输出和营养物质的供应来实现的。此外，AEP在操纵肠道菌群方面比WEP更关键。
The purpose of this study was to investigate the effect of okra polysaccharides with similar molecular weight on intestinal ecology. After extraction, ethanol precipitation, deproteinization and purification, two polysaccharide fractions, named as water-extracted polysaccharides (WEP) and alkaline-extracted polysaccharides (AEP), were obtained. According to the results of high performance liquid chromatography, AEP contained mannose, rhamnose, galacturonic acid and galactose, while WEP was composed of galactose, rhamnose, fructose, arabinose, glucose, galacturonic acid and mannose. The compositions of intestinal microbiota were affected by the change of daily substrates. Meanwhile, the concentration of ammonia and gastrointestinal transit time decreased, the total short chain fatty acid (SCFA) levels, moisture of caecum content increased. These findings suggested that the effect of okra polysaccharides on intestinal microbiota was achieved by the output of detrimental compounds and the supply of nutrients. Besides, AEP supported a more pivotal role in manipulating gut microbiota than WEP.