HeLa cells and their possible contamination of other cell lines: karyotype studies.
HeLa cells and their possible contamination of other cell lines: karyotype studies.
复制标题
HeLa 细胞及其对其他细胞系的可能污染：核型研究。
DOI：
10.1111/j.1601-5223.1976.tb01560.x
复制
发表时间：
2009
期刊：
影响因子：
2.7
通讯作者：
W. Heneen
中科院分区：
文献类型：
--
作者：
W. Heneen
G 和 C 带技术应用于 5 种人类细胞系的染色体，即HeLa、KB、HEp-2、D98/AH-2 和 Lu106。据记载，前3例均为肿瘤来源。后者是正常来源的。这5个细胞系表现出相当大的相似性：（1）它们的干系染色体数目接近三倍体； (2)正常染色体类型。通常以二体性或三体性数量存在，在 4 个或所有 5 个品系中显示出相同的偏差：第 3 号为无效或单体性，第 7、15 和 17 号为三体或四体性，第 7、9 和 16 号为最大变异，尽管某些品系被认为是雄性起源，但没有 Y 染色体； (3) 标记物的发生率大小顺序相当（KB、HeLa 和 D98/AH-2 中为 21-25%，HEp-2 中为 29%，Lu106 中为 36%）； (4) 大多数标记类型的发现频率相对较低或较高（10 个细胞中有 1-3 或 8-10 个）； (5)不同品系间具有一致带型的标记染色体是共有的； (6)大多数或所有品系中正常和标记染色体的总长度以及整个互补体大致相同。这些结果表明，在完全独立的品系中，所分析的参数的变异性小于预期。结论是所有 5 种细胞系最有可能源自 HeLa。
G- and C-banding techniques were applied to the chromosomes of 5 human cell lines, viz. HeLa, KB, HEp-2, D98/AH-2 and Lu106. According to the records, the 3 former ones are of tumor origin. the 2 latter of normal origin. The 5 cell lines showed considerable similarities: (1) their stemline chromosome numbers were near-triploid; (2) the normal chromosome types. generally present in disomic or trisomic numbers, showed the same deviations through 4 or all 5 lines: nulli- or monosomy in No. 3, tri- or tetrasomy in No. 7, 15 and 17, maximum variation in No. 7, 9 and 16, no Y chromosome in spite of the supposedly male origin of some of the lines; (3) the incidence of markers was of comparable size order (21–25% in KB, HeLa and D98/AH-2, 29% in HEp-2, 36% in Lu106); (4) the majority of the marker types were found either in relatively low or high frequencies (1–3 or 8–10 out of 10 cells); (5) marker chromosomes of concordant band pattern were common to different lines; (6) the total lengths of normal and marker chromosomes and whole complements were approximately the same in most or all lines. These results indicate that the variability in the parameters analyzed is less than expected in completely independent lines. The conclusion is that all 5 cell lines are most likely of HeLa origin.