Blue and cyan fluorescent carbon dots: one-pot synthesis, selective cell imaging and their antiviral activity
Blue and cyan fluorescent carbon dots: one-pot synthesis, selective cell imaging and their antiviral activity
复制标题
蓝色和青色荧光碳点：一锅合成、选择性细胞成像及其抗病毒活性
DOI：
10.1039/c7ra03167j
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Xiao, Shaobo
中科院分区：
文献类型：
--
作者：
Liu, Huabing;Bai, Yanli;Xiao, Shaobo
碳点(Cd)在细胞成像和跟踪方面显示出诱人的潜力。然而，最具挑战性的问题之一是CDS选择性地进入细胞质或细胞核。在这里，发展了一种简便的方法来制备两种类型的镉，它们能够选择性地进入细胞质和整个细胞，而不需要进一步的表面修饰。水热过程中加入尿素后，硫化镉的表面电荷由负电荷变为中性，制备的硫化镉呈现出不同的荧光颜色。蓝光CDS(b-CDS)只能进入细胞质，而蓝光CDS(c-CDS)可以分布于整个细胞，包括细胞核。此外，还研究了这两种CD对伪狂犬病病毒(PRV)的抗病毒活性，发现b-CD具有更好的抗病毒活性。本研究为制备用于选择性细胞成像的硫化镉提供了一种简便、新颖的策略，为硫化镉在纳米医学中的应用提供了可能。
Carbon dots (CDs) have demonstrated attractive potential in cell imaging and tracking. However, one of the most challenge problems is the selective entrance of CDs into the cytoplasm or cell nucleus. Here, a facile strategy was developed to prepare two types of CDs capable of selective entrance into cytoplasm and the whole cell without further surface modification. The surface charge of CDs changes from negative to neutral after the addition of urea in the hydrothermal process, and the as-prepared CDs exhibit different fluorescent colors. The blue-fluorescent CDs (b-CDs) can only enter the cytoplasm, while the cyan-fluorescent CDs (c-CDs) can distribute over the whole cell, including the nucleus. Additionally, the antiviral activity of the two CDs against pseudorabies virus (PRV) was studied and b-CDs were shown to have better antiviral activity. This study has provided a facile and novel strategy for the fabrication of CDs used in selective cell imaging, which may facilitate the application of CDs in nanomedicine.