Role of nsdA in negative regulation of antibiotic production and morphological differentiation in Streptomyces bingchengensis
Role of nsdA in negative regulation of antibiotic production and morphological differentiation in Streptomyces bingchengensis
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DOI：
10.1038/ja.2009.33
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发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Xiang, Wen-Sheng
中科院分区：
文献类型：
--
作者：
Wang, Xiang-Jing;Guo, Suo-Lian;Xiang, Wen-Sheng
为研究nsdA基因在宾城链霉菌中的功能，克隆了该基因，并进行了序列测定。天蓝色。使用λ RED介导的PCR靶向技术在S. bingchengensis_226541染色体。获得nsdA破坏突变体BC 29，其产生比祖先菌株更多的色素和孢子。高效液相色谱分析表明，nsdA的破坏有效地提高了米尔贝霉素A（4）和南昌霉素的产量分别为1.5倍和9倍。nsdA突变的互补恢复了表型和抗生素生产。这些结果表明，nsdA对S.冰城种抗生素杂志（2009）62，309-313; doi：10.1038/ja.2009.33; 2009年5月15日在线发表
To investigate the function of nsdA in Streptomyces bingchengensis, it was cloned and sequenced, which presented an 89.89% identity with that of S. coelicolor. The lambda RED-mediated PCR-targeting technique was used to create nsdA replacement in the S. bingchengensis_226541 chromosome. The nsdA disruption mutant, BC29, was obtained, which produced more pigment and spores than did the ancestral strain. HPLC analysis revealed that the disruption of nsdA efficiently increased milbemycin A(4) production and nanchangmycin production by 1.5-fold and 9-fold, respectively. Complementation of the nsdA mutation restored the phenotype and antibiotic production. These results showed that nsdA negatively affected sporulation and antibiotic production in S. bingchengensis. The Journal of Antibiotics (2009) 62, 309-313; doi:10.1038/ja.2009.33; published online 15 May 2009