Bovine mitochondrial peroxiredoxin III forms a two-ring catenane
Bovine mitochondrial peroxiredoxin III forms a two-ring catenane
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DOI：
10.1016/j.str.2005.07.021
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发表时间：
2005-11-01
期刊：
影响因子：
5.7
通讯作者：
Isaacs, NW
中科院分区：
文献类型：
--
作者：
Cao, ZB;Roszak, AW;Isaacs, NW
晶体结构的C168 S突变体的一个典型的2-半胱氨酸过氧化物酶III（Prx III）从牛线粒体的分辨率为3.3埃的报告。Prx III作为双环索烃存在，其包含由基本二聚体单元组装的两个互锁十二聚体环。每个环具有150埃的外径，并包围宽度为70埃的中心腔。串联的十二聚体以55度的角度倾斜，这在环之间提供了大的接触表面。环形成中涉及的二聚体-二聚体接触本质上是疏水性的，而互锁环之间的12个接触区域来自极性相互作用。这两种主要的亚基相互作用模式提供了重要的见解可能的机制索烃形成。
A crystal structure is reported for the C168S mutant of a typical 2-Cys peroxiredoxin III (Prx III) from bovine mitochondria at a resolution of 3.3 angstrom. Prx III is present as a two-ring catenane comprising two interlocking dodecameric toroids that are assembled from basic dimeric units. Each ring has an external diameter of 150 A and encompasses a central cavity that is 70 angstrom in width. The concatenated dodecamers are inclined at an angle of 55 degrees, which provides a large contact surface between the rings. Dimer-dimer contacts involved in toroid formation are hydrophobic in nature, whereas the 12 areas of contact between interlocked rings arise from polar interactions. These two major modes of subunit interaction provide important insights into possible mechanisms of catenane formation.