Quantitative PA tomography of high resolution 3-D images: Experimental validation in a tissue phantom
Quantitative PA tomography of high resolution 3-D images: Experimental validation in a tissue phantom
复制标题
DOI：
10.1016/j.pacs.2019.100157
复制
发表时间：
2020-03-01
期刊：
影响因子：
7.9
通讯作者：
Laufer, Jan
中科院分区：
文献类型：
--
作者：
Buchmann, Jens;Kaplan, Bernhard;Laufer, Jan
定量光声层析成像的目的是从深层组织的高分辨率图像中恢复绝对发色团浓度及其比例的空间分布。在这项研究中，一个基于模型的反演方案的基础上的蒙特-卡罗光传输模型进行实验验证的3-D多光谱图像的组织幻影收购使用全光学扫描仪与平面检测几何形状。一个校准的吸收器允许在反演过程中的测量数据的缩放，而声学校正方法被用来补偿有限的视图检测的影响。生色团和能量密度依赖的步长和亚当优化实现快速收敛。高分辨率的3-D地图的绝对浓度和它们的比例恢复了高精度。该方法的潜在应用包括在临床前和临床研究中的深部组织的定量功能和分子光声断层扫描。
Quantitative photoacoustic tomography aims to recover the spatial distribution of absolute chromophore concentrations and their ratios from deep tissue, high-resolution images. In this study, a model-based inversion scheme based on a Monte-Carlo light transport model is experimentally validated on 3-D multispectral images of a tissue phantom acquired using an all-optical scanner with a planar detection geometry. A calibrated absorber allowed scaling of the measured data during the inversion, while an acoustic correction method was employed to compensate the effects of limited view detection. Chromophore- and fluence-dependent step sizes and Adam optimization were implemented to achieve rapid convergence. High resolution 3-D maps of absolute concentrations and their ratios were recovered with high accuracy. Potential applications of this method include quantitative functional and molecular photoacoustic tomography of deep tissue in preclinical and clinical studies.