Expression of estrogen receptor β is developmentally regulated in reproductive tissues of male and female mice
Expression of estrogen receptor β is developmentally regulated in reproductive tissues of male and female mice
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DOI：
10.1095/biolreprod62.2.310
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发表时间：
2000-02-01
影响因子：
3.6
通讯作者：
Newbold, RR
中科院分区：
文献类型：
--
作者：
Jefferson, WN;Couse, JF;Newbold, RR
应用核糖核酸酶保护试验（RPA）结合免疫组织化学技术，对雌、雄小鼠胚胎14天至出生后26天（PND 26）性腺和生殖道雌激素受体（ER）和雌激素受体（ERP）的表达进行了定位。在卵巢中，ER α mRNA检测在所有年龄检查; ER β mRNA被认为是早在PND 1，其表达随年龄增加。免疫定位显示ER β在卵巢颗粒细胞分化，而ER α主要见于间质细胞。其余的女性生殖道显示ER α mRNA在所有年龄检查很少或没有显着水平的ER β，除了在END 1时，出现低水平的消息。在雄性动物中，在胎儿睾丸中检测到ER α和ERP mRNA;然而，ER β逐渐增加，直至PND 5，随后在PND 26时降至不可检测水平。免疫定位显示，ER α在睾丸间质室，而ER β主要见于发育中的精原细胞，其余的男性生殖道显示出不同量的RPA和免疫染色在整个发育过程中的受体。这些研究提供了有用的信息，在研究ER亚型在正常分化中的作用，他们提供了发展过程中的差异组织表达的迹象。
By the use of ribonuclease protection assay (RPA) combined with immunohistochemical techniques, the expression of estrogen receptor (ER) or and ERP was mapped in the developing gonads and reproductive tracts of male and female mice from fetal day 14 to postnatal day 26 (PND 26), This study was designed to determine the pattern of expression of both ER subtypes in specific tissue compartments during development. In ovaries, ER alpha mRNA was detected at all ages examined; ER beta mRNA was seen as early as PND 1, and its expression increased with age. Immunolocalization showed ER beta in differentiating granulosa cells of the ovary, whereas ER alpha was predominantly seen in interstitial cells. The remainder of the female reproductive tract showed ER alpha mRNA at all ages examined with little or no significant levels of ER beta, except on END 1 when a low level of message appeared. In males, ER alpha and ERP mRNA were detected in the fetal testis; however, ER beta gradually increased until PND 5 and subsequently diminished to undetectable levels by PND 26. Immunolocalization showed ER alpha in the interstitial compartment of the testis, whereas ER beta was seen predominantly in developing spermatogonia, The remainder of the male reproductive tract showed varying amounts of both receptors by RPA and immunostaining throughout development. These studies provide information useful in studying the role of both ER subtypes in normal differentiation, and they provide indications of differential tissue expression during development.