Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development
Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development
复制标题
DOI：
10.1016/s1097-2765(00)80022-2
复制
发表时间：
1998-01-01
期刊：
影响因子：
16
通讯作者：
Deng, XW
中科院分区：
文献类型：
--
作者：
Ang, LH;Chattopadhyay, S;Deng, XW
拟南芥 COP1 作为光形态发生发育的光失活抑制因子，但其分子作用模式仍不清楚。在这里，我们发现 COP1 负向调节 HY5，这是一种 bZIP 蛋白，也是光形态发育的正向调节因子。体外和体内测定均表明 COP1 与 HY5 直接且特异性地相互作用。由缺乏 COP1 相互作用结构域的突变体 HY5 过度表达引起的超光形态发生表型支持 HY5-COP1 相互作用的调节作用。此外，HY5 能够直接与 CHS1 最小启动子相互作用，并且对其光激活至关重要。我们认为COP1与转录因子的直接相互作用和对转录因子的调节可能代表了其控制基因表达和光形态发生发育的分子机制。
Arabidopsis COP1 acts as a light-inactivable repressor of photomorphogenic development, but its molecular mode of action remains unclear. Here, we show that COP1 negatively regulates HY5, a bZIP protein and a positive regulator of photomorphogenic development. Both in vitro and in vivo assays indicate that COP1 interacts directly and specifically with HY5. The hyper-photomorphogenic phenotype caused by the overexpression of a mutant HY5, which lacks the COP1-interactive domain, supports the regulatory role of HY5-COP1 interaction. Further, HY5 is capable of directly interacting with the CHS1 minimal promoter and is essential for its light activation. We propose that the direct interaction with and regulation of transcription factors by COP1 may represent the molecular mechanism for its control of gene expression and photomorphogenic development.