Mechanical separation of the complementary strands of DNA
Mechanical separation of the complementary strands of DNA
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DOI：
10.1073/pnas.94.22.11935
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发表时间：
1997-10-28
影响因子：
11.1
通讯作者：
Heslot, F
中科院分区：
文献类型：
--
作者：
EssevazRoulet, B;Bockelmann, U;Heslot, F
我们描述了一个单一的噬菌体λ DNA分子的两个互补链的机械分离。分子一端的3'端和5'端逐渐被拉开，这导致双螺旋的打开。沿着开口的典型力在10-15 pN的范围内。分离力信号被示出为与局部GC相对于AT含量沿着分子。目前检测到该含量在100-500个碱基的典型规模上的变化。
We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.