Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera
Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera
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DOI：
10.1016/j.virol.2005.10.032
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发表时间：
2006-03-01
期刊：
影响因子：
3.7
通讯作者：
Hauschild, W
中科院分区：
文献类型：
--
作者：
Jehle, JA;Lange, M;Hauschild, W
通过扩增高度保守的杆状病毒基因晚期表达因子8(lef-8)、晚期表达因子9(lef-9)和多角体蛋白/颗粒蛋白(polh/gran)，结合分子系统发育分析，为鳞翅目昆虫特有的杆状病毒的鉴定和多样性研究提供了有力的工具。在目前的研究中，我们改进了简并寡核苷酸，并证实了Lange等人最近描述的方法。(Lange，M.，Wang，H.，Zhihong，H.，Jehle，J.A.，2004。建立鳞翅目昆虫特有杆状病毒的分子鉴定和分类系统。病毒学325、36-47)。从71份历史上未鉴定的杆状病毒标本中提取杆状病毒DNA，用基因特异性简并聚合酶链式反应扩增出部分基因序列。获得的PCR产物直接测序，推导的氨基酸序列被编译并与这些目的基因的已发表序列进行比对。用最大简约法和距离法建立了117种杆状病毒的系统发育树。基于对部分lef-8、lef-9和polh/gran基因的系统发育分析，我们提出了鳞翅目特有杆状病毒的系统发育种标准，该标准使用这些基因的遗传距离来划分物种。(C)2005 Elsevier Inc.保留所有权利。
PCR amplification of the highly conserved baculovirus genes late expression factor 8 (lef-8), late expression factor 9 (lef-9) and polyhedrin/granulin (polh/gran) combined with molecular phylogenetic analyses provide a powerful tool to identify lepidopteran-specific baculoviruses and to study their diversity. In the present investigation, we have improved the degenerate oligonucleotides and corroborated the approach that was recently described by Lange et al. (Lange, M., Wang, H., Zhihong, H., Jehle, J.A., 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology 325, 36-47.). Baculovirus DNA was isolated from 71 uncharacterized historic baculovirus samples, and partial gene sequences were amplified by using gene-specific degenerate PCR primers. The obtained PCR products were directly sequenced, and the deduced amino acid sequences were compiled and aligned with published sequences of these target genes. A phylogenctic tree of 117 baculoviruses was inferred using maximum parsimony and distance methods. Based on the comprehensive phylogenetic analysis of the partial lef-8, lef-9 and polh/gran genes, we propose a phylogenetic species criterion for lepidopteran-specific baculoviruses that uses the genetic distances of these genes for species demarcation. (c) 2005 Elsevier Inc. All rights reserved.