Protective effects of hydrogen gas against spinal cord ischemia-reperfusion injury
Protective effects of hydrogen gas against spinal cord ischemia-reperfusion injury
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DOI：
10.1016/j.jtcvs.2021.04.077
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发表时间：
2022-11-11
影响因子：
6
通讯作者：
Mori, Takashi
中科院分区：
文献类型：
--
作者：
Kimura, Aya;Suehiro, Koichi;Mori, Takashi
目的：本实验旨在通过体内微透析法测定脊髓前角谷氨酸浓度，评估氢气吸入对脊髓缺血再灌注损伤的疗效并揭示其作用机制。方法：将雄性Sprague-Dawley大鼠分为以下6组：假手术、仅脊髓缺血、3%氢气（脊髓缺血刺3%氢气）、2%氢气（脊髓缺血刺2%氢气）、1%氢气（脊髓缺血刺1%氢气）、气氢二氢酸盐（脊髓缺血刺二氢酸盐[谷氨酸转运蛋白选择性抑制剂-1]刺3%氢气）。缺血前10分钟开始吸入氢气。二氢盐组在缺血前20分钟给予谷氨酸转运蛋白-1抑制剂。免疫荧光法检测谷氨酸转运蛋白-1在大鼠腹角的表达。结果：3%氢气吸入可明显抑制脊髓缺血所致细胞外谷氨酸的升高
Objective: This experimental study aimed to assess the efficacy of hydrogen gas inhalation against spinal cord ischemia-reperfusion injury and reveal its mechanism by measuring glutamate concentration in the ventral horn using an in vivo microdialysis method.Methods: Male Sprague-Dawley rats were divided into the following 6 groups: sham, only spinal ischemia, 3% hydrogen gas (spinal ischemia thorn 3% hydrogen gas), 2% hydrogen gas (spinal ischemia thorn 2% hydrogen gas), 1% hydrogen gas (spinal ischemia thorn 1% hydrogen gas), and hydrogen gas dihydrokainate (spinal ischemia thorn dihydrokainate [selective inhibitor of glutamate transporter-1] thorn 3% hydrogen gas). Hydrogen gas inhalation was initiated 10 minutes before the ischemia. For the hydrogen gas dihydrokainate group, glutamate transporter-1 inhibitor was administered 20 minutes before the ischemia. Immunofluorescence was performed to assess the expression of glutamate transporter-1 in the ventral horn.Results: The increase in extracellular glutamate induced by spinal ischemia was significantly suppressed by 3% hydrogen gas inhalation (P