Neuronally mediated and direct effects of prostaglandins on ion transport in rat colon descendens
Neuronally mediated and direct effects of prostaglandins on ion transport in rat colon descendens
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前列腺素对大鼠结肠下行离子转运的神经介导和直接影响
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
W. Rummel
中科院分区：
文献类型：
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作者：
M. Diener;R. Bridges;S. Knobloch;W. Rummel
本实验采用两种大鼠降结肠标本，对伊洛前列素和前列腺素E_2（PGE_2）的作用部位进行了定位研究。一种制备物，粘膜-粘膜下层制备物含有粘膜下层和粘膜丛，而对于另外的粘膜制备物，除去具有粘膜下层丛的粘膜下层。伊洛前列素（10−6 mol · 1−1）导致粘膜-粘膜下层制备物的短路电流（Isc）、电位差（Pd）和组织电导（Gt）增加，反映了单向离子通量测量证实的净Cl−分泌。Cl−分泌是由于JinfsmsupCl增加和JinfmssupCl减少所致。加入5 × 10 - 5 mol · l-1阿托品可完全消除上述效应。伊洛前列素在粘膜制备中仅具有较小且不一致的作用。相比之下，PGE 2（10 - 6 mol · l-1）由于两种制剂中的Cl-分泌而增加了Isc、Pd和Gt。Cl−分泌是由JinfsmsupCl增加和JinfmsCl减少引起的，阿托品仅抑制PGE 2在粘膜-粘膜下层制剂中的作用，而不抑制粘膜制剂中的作用。结果表明，前列环素衍生物伊洛前列素仅通过激活粘膜下神经元诱导Cl−分泌，而PGE 2作用于上皮和粘膜下神经丛。胡枝子素的神经元效应似乎至少部分由毒蕈碱受体介导。
SummaryTwo preparations of rat colon descendens were used in order to localize the action sites of iloprost and prostaglandin E2 (PGE2). One preparation, the mucosa-submucosa preparation contained the submucosal and mucosal plexus whereas for the mucosa preparation in addition the submucosa with the submucosal plexus was removed. Iloprost (10−6 mol · 1−1) caused an increase in short-circuit current (Isc), potential difference (Pd) and tissue conductance (Gt) of the mucosa-submucosa preparation reflecting net Cl− secretion as confirmed by unidirectional ion flux measurements. The Cl− secretion was due to an increase in JinfsmsupCland a decrease in JinfmssupCl. These effects were completely abolished by addition of 5 × 10−5 mol · l−1 atropine. Iloprost had only small and inconsistent effects in the mucosa preparation. In contrast PGE2 (10−6 mol · l−1) increased Isc, Pd and Gt due to Cl− secretion in both preparations. The Cl− secretion was caused by an increase in JinfsmsupCland a decrease in JinfmsClOnly the PGE2 effect in the mucosa-submucosa preparation but not in the mucosa preparation was inhibited by about 50% by atropine. The results suggest that the prostacyclin derivative iloprost induces a Cl− secretion only by an activation of submucosal neurons whereas PGE2 acts both on the epithelium and the submucosal plexus. The neuronal effects of prostaglandins appear to be, at least in part, mediated by muscarinic receptors.
体外人回肠离子转运：电场刺激和河豚毒素的变化。
影响因子：
29.4
作者：
Hubel,KA;Shirazi,S
通讯作者：
Shirazi,S
与残余结肠相比，分离的浅表和增殖性结肠上皮细胞中前列腺素合成和降解的模式。
DOI：
10.1016/0090-6980(83)90196-x
发表时间：
1983
期刊：
Prostaglandins
影响因子：
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作者：
Craven,PA;DeRubertis,FR
通讯作者：
DeRubertis,FR