Magnetoresistive-based real-time cell phagocytosis monitoring.
Magnetoresistive-based real-time cell phagocytosis monitoring.
复制标题
基于磁阻的实时细胞吞噬监测。
DOI：
10.1016/j.bios.2012.04.002
复制
发表时间：
2012
影响因子：
12.6
通讯作者：
H. Brueckl
中科院分区：
文献类型：
--
作者：
A. Shoshi;J. Schotter;P. Schroeder;M. Milnera;P. Ertl;V. Charwat;M. Purtscher;R. Heer;M. Eggeling;G. Reiss;H. Brueckl
细胞对大颗粒的摄取（吞噬作用）是细胞生物学中的一个重要因素，在生物医学应用中也起着重要作用。到目前为止，大多数用于确定吞噬特性的方法依赖于细胞培养孵育和终点检测方案。在这里，我们提出了一个实验室上的一个芯片系统，用于实时监测磁性颗粒摄取的人成纤维细胞（NHDF）。它是基于巨磁阻（GMR）型传感器记录吞噬过程中磁性颗粒的平均位置和分布的时间演变。我们采用平均直径为1.2μm的颗粒，并表征其吞噬相关性质。我们在生理条件下的实验显示，细胞摄取速率为每小时45个颗粒，并表明吞噬作用在平均摄取时间27.7小时后达到饱和。此外，在4°C下进行参考吞噬实验以模拟环境或疾病相关的吞噬行为抑制，我们的测量清楚地表明我们能够区分细胞膜粘附和吞噬的磁性颗粒。除了所展示的吞噬机制的实时监测之外，还可以实现额外的纳米生物界面研究，包括芯片上细胞粘附/扩散以及细胞迁移、附着和分离动力学。这种多功能性显示了我们的方法为芯片上细胞分析提供多功能平台的潜力。
The uptake of large particles by cells (phagocytosis) is an important factor in cell biology and also plays a major role in biomedical applications. So far, most methods for determining the phagocytic properties rely on cell-culture incubation and end-point detection schemes. Here, we present a lab-on-a-chip system for real-time monitoring of magnetic particle uptake by human fibroblast (NHDF) cells. It is based on recording the time evolution of the average position and distribution of magnetic particles during phagocytosis by giant-magnetoresistive (GMR) type sensors. We employ particles with a mean diameter of 1.2μm and characterize their phagocytosis-relevant properties. Our experiments at physiological conditions reveal a cellular uptake rate of 45 particles per hour and show that phagocytosis reaches saturation after an average uptake time of 27.7h. Moreover, reference phagocytosis experiments at 4°C are carried out to mimic environmental or disease related inhibition of the phagocytic behavior, and our measurements clearly show that we are able to distinguish between cell-membrane adherent and phagocytosed magnetic particles. Besides the demonstrated real-time monitoring of phagocytosis mechanisms, additional nano-biointerface studies can be realized, including on-chip cell adhesion/spreading as well as cell migration, attachment and detachment dynamics. This versatility shows the potential of our approach for providing a multifunctional platform for on-chip cell analysis.
影响因子：
3.4
作者：
Holevinsky, KO;Nelson, DJ
通讯作者：
Nelson, DJ