Different antiviral activities of natural APOBEC3C, APOBEC3G, and APOBEC3H variants against hepatitis B virus
Different antiviral activities of natural APOBEC3C, APOBEC3G, and APOBEC3H variants against hepatitis B virus
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DOI：
10.1016/j.bbrc.2019.08.003
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发表时间：
2019-10-08
影响因子：
3.1
通讯作者：
Kitamura, Kouichi
中科院分区：
文献类型：
--
作者：
Kanagaraj, Arun;Sakamoto, Naoya;Kitamura, Kouichi
一些APOBEC3家族成员对逆转录病毒和DNA病毒具有抗病毒活性。乙型肝炎病毒（HBV）是一种DNA病毒，是肝硬化和肝细胞癌等严重肝脏疾病的主要致病因素。为了确定人类APOBEC3变体是否具有不同的抗hbv活性，我们使用hbv复制细胞培养模型评估了APOBEC3C、APOBEC3G和APOBEC3H的自然变体。我们的数据表明，APOBEC3C变体S188I对HBV DNA具有增加的限制性活性和高突变频率。相比之下，APOBEC3G变体H186R没有改变抗hbv和高突变活性。在APOBEC3H多态性（hap I-VII）和剪接变体（SV-200、SV-183、SV-182和SV-154）中，hap II SV-183表现出最强的限制性活性。这些数据提示APOBEC3基因的遗传变异可能影响人类清除HBV的效率。(C) 2019 Elsevier Inc.版权所有。
Some APOBEC3 family members have antiviral activity against retroviruses and DNA viruses. Hepatitis B virus (HBV) is a DNA virus that is the major causative factor of severe liver diseases such as cirrhosis and hepatocellular carcinoma. To determine whether APOBEC3 variants in humans have different anti-HBV activities, we evaluated natural variants of APOBEC3C, APOBEC3G, and APOBEC3H using an HBV-replicating cell culture model. Our data demonstrate that the APOBEC3C variant S188I had increased restriction activity and hypermutation frequency against HBV DNA. In contrast, the APOBEC3G variant H186R did not alter the anti-HBV and hypermutation activities. Among APOBEC3H polymorphisms (hap I-VII) and splicing variants (SV-200, SV-183, SV-182, and SV-154), hap II SV-183 showed the strongest restriction activity. These data suggest that the genetic variations in APOBEC3 genes may affect the efficiency of HBV elimination in humans. (C) 2019 Elsevier Inc. All rights reserved.