Activation of PRK1 by Phosphatidylinositol 4,5-Bisphosphate and Phosphatidylinositol 3,4,5-Trisphosphate
Activation of PRK1 by Phosphatidylinositol 4,5-Bisphosphate and Phosphatidylinositol 3,4,5-Trisphosphate
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磷脂酰肌醇 4,5-二磷酸和磷脂酰肌醇 3,4,5-三磷酸激活 PRK1
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
P. Parker
中科院分区：
文献类型：
--
作者：
R. Palmer;L. Dekker;R. Woscholski;J. Good;R. Gigg;P. Parker
作为多磷酸肌醇的潜在靶标，蛋白激酶 C (PKC) 同种型（β1、ε、ze、η）和 PKC 相关激酶 (PRK) 家族成员 PRK1 的激活已在体外进行了比较。 PRK1 可被磷脂酰肌醇 4,5-二磷酸 (PtdIns 4,5-P2) 和磷脂酰肌醇 3,4,5-三磷酸 (PtdIns-3,4,5-P3) 激活，无论是作为纯超声处理脂质还是在洗涤剂混合胶束中。当以超声处理的脂质形式存在时，PtdIns-4,5-P2 和 PtdIns-3,4,5-P3 在激活 PRK1 方面是等效的，此外，超声处理的磷脂酰肌醇 (PtdIns) 和磷脂酰丝氨酸 (PtdSer) 也同样有效。在洗涤剂混合胶束中，PtdIns-4,5-P2 和 PtdIns-3,4,5-P3 也显示出相似的效力，但 PtdIns 和 PtdSer 在此测定中的有效性低 10 倍。类似地，洗涤剂混合胶束中的 PKC-β1、-ε 和 -η 均被 PtdIns-4,5-P2 和 PtdIns-3,4,5-P3 激活。对于所有测试的激酶，PtdIns-4,5-P2 和 PtdIns-3,4,5-P3 的激活常数基本相同，这意味着该体外分析没有特异性。与该结论一致，发现 PtdIns-4,5-P2 和 PtdIns-3,4,5-P3 的作用在 10 mM Mg2+ 下受到抑制，并被高浓度的肌醇六磷酸盐和肌醇六硫酸盐所模拟。根据它们作为第二信使的潜在作用，讨论了这两类脂质激活蛋白激酶对这些磷酸肌醇的相似反应。
As potential targets for polyphosphoinositides, activation of protein kinase C (PKC) isotypes (β1, ε, ζ, η) and a member of the PKC-related kinase (PRK) family, PRK1, has been compared in vitro. PRK1 is shown to be activated by both phosphatidylinositol 4,5-bisphosphate (PtdIns 4,5-P2) as well as phosphatidylinositol 3,4,5-trisphosphate (PtdIns-3,4,5-P3) either as pure sonicated lipids or in detergent mixed micelles. When presented as sonicated lipids, PtdIns-4,5-P2 and PtdIns-3,4,5-P3 were equipotent in activating PRK1, and, furthermore, sonicated phosphatidylinositol (PtdIns) and phosphatidylserine (PtdSer) were equally effective. In detergent mixed micelles, PtdIns-4,5-P2 and PtdIns-3,4,5-P3 also showed a similar potency, but PtdIns and PtdSer were 10-fold less effective in this assay. Similarly, PKC-β1, -ε, and -η were all activated by PtdIns-4,5-P2 and PtdIns-3,4,5-P3 in detergent mixed micelles. The activation constants for PtdIns-4,5-P2 and PtdIns-3,4,5-P3 were essentially the same for all the kinases tested, implying no specificity in this in vitro analysis. Consistent with this conclusion, the effects of PtdIns-4,5-P2 and PtdIns-3,4,5-P3 were found to be inhibited at 10 mM Mg2+ and mimicked by high concentrations of inositol hexaphosphate and inositol hexasulfate. The similar responses of these two classes of lipid-activated protein kinase to these phosphoinositides are discussed in light of their potential roles as second messengers.
含有二酰甘油和磷脂酰丝氨酸的 Triton X-100 混合胶束激活蛋白激酶 C。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hannun,YA;Loomis,CR;Bell,RM
通讯作者：
Bell,RM
磷脂酰肌醇 3,4,5-三磷酸激活蛋白激酶 C。
影响因子：
3.1
作者：
Singh,SS;Chauhan,A;Brockerhoff,H;Chauhan,VP
通讯作者：
Chauhan,VP