Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12
Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12
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大肠杆菌 K-12 中核酸外切酶 III 的结构基因 xthA 的遗传图谱
DOI：
10.1128/jb.126.3.1082-1088.1976
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发表时间：
1976
影响因子：
3.2
通讯作者：
B. Weiss
中科院分区：
文献类型：
--
作者：
B. White;S. Hochhauser;N. Cintrón;B. Weiss
基因xthA，pncA，和pabB相对于其他人的两个和三个因素的转导杂交与噬菌体P1。所研究的基因在大肠杆菌K-12的顺时针序列pheS-pfkB-xthA-pncA-gap-pabB-fadD的遗传图谱中跨越2分钟（2%）。11个独立衍生的xth突变进行了检查，所有已知的影响核酸外切酶III和其相关的核酸内切酶II的活性，并在xthA区域映射。发现pncA突变赋予对6-氨基烟酰胺的抗性，而已知一些pheS突变指定对氟苯丙氨酸的抗性。通过选择这些可共转导的药物抗性标记，XTH突变很容易转移到其它菌株中。
The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.