DEMONSTRATION OF B-CELL MATURATION IN X-LINKED IMMUNODEFICIENT MICE BY SIMULTANEOUS 3-COLOR IMMUNOFLUORESCENCE
DEMONSTRATION OF B-CELL MATURATION IN X-LINKED IMMUNODEFICIENT MICE BY SIMULTANEOUS 3-COLOR IMMUNOFLUORESCENCE
复制标题
DOI：
10.1038/306270a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
HERZENBERG, LA
中科院分区：
文献类型：
--
作者：
HARDY, RR;HAYAKAWA, K;HERZENBERG, LA
携带X连锁免疫缺陷基因（xid）的CBA/N小鼠的脾B细胞比正常CBA小鼠少，并且对某类抗原无反应1。对B细胞表面标志物表达2和免疫应答3的研究已经导致了一种普遍接受的观点，即成年小鼠中的B细胞是不成熟的，与年轻（1-3周龄）正常小鼠的B细胞相似。也就是说，像年轻的动物一样，xid小鼠缺乏成年脾脏中存在的三种IgM/IgD B细胞亚群中最多的细胞（在图1a，B中指定为I）4，5。然而，我们现在报告说，这种情况过于简单化，事实上，成年小鼠的B细胞不同于成年或年轻的正常小鼠。应用三色荧光激活细胞分选仪（FACS）定量分析，比较了正常和氧化小鼠脾B细胞上IgM、IgD和一种新发现的B淋巴细胞抗原（BLA-1）的相关表达。我们发现：（1）成年氧化小鼠（和正常小鼠一样）的大多数B细胞是BLA-1−，而幼年动物的所有B细胞都是BLA-1+;（2）氧化小鼠和正常小鼠之间IgM/IgD B细胞亚群的主要差异仅限于BLA-1−细胞;（3）氧化小鼠的BLA-1+ III群B细胞数量增加。
CBA/N mice carrying the X-linked immune deficiency gene (xid) have fewer splenic B cells than normal CBA mice and are unresponsive to a certain class of antigens1. Studies of B-cell surface-marker expression2and immune responsiveness3have led to the commonly accepted idea that the B cells in adultxidmice are immature and resemble the B cells of young (1–3 week old) normal mice. That is, like young animals,xidmice lack cells in the most numerous of three IgM/IgD B-cell sub-populations (designated I in Fig. 1a,b) present in adult spleen4,5. We now report, however, that this picture is an oversimplification and that in fact the B cells in adultxidmice differ from those present in either adult or young normal mice. Using quantitative three-colour fluorescence-activated cell sorter (FACS) analyses, we have compared the correlated expression of IgM, IgD and a newly discovered B-lymphocyte antigen (BLA-1) on splenic B cells in normal andxidmice. We show here (1) that most B cells in adultxidmice (as in normals) are BLA-1−whereas all B cells in young animals are BLA-1+; (2) that the major difference in the IgM/IgD B-cell subpopulations found betweenxidand normal mice is limited to the BLA-1−cells; and (3) thatxidmice have increased numbers of BLA-1+population III B cells.