AROMATIC L-AMINO-ACID DECARBOXYLASE ACTIVITY OF MOUSE STRIATUM IS MODULATED VIA DOPAMINE-RECEPTORS
AROMATIC L-AMINO-ACID DECARBOXYLASE ACTIVITY OF MOUSE STRIATUM IS MODULATED VIA DOPAMINE-RECEPTORS
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DOI：
10.1111/j.1471-4159.1993.tb03503.x
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发表时间：
1993-06-01
影响因子：
4.7
通讯作者：
NEFF, NH
中科院分区：
文献类型：
--
作者：
HADJICONSTANTINOU, M;WEMLINGER, TA;NEFF, NH
在给予单剂量多巴胺受体拮抗剂氟哌啶醇、舒必利后，对照小鼠和MPTP处理小鼠的纹状体中芳香族L-氨基酸脱羧酶(AAAD)活性增强。和 SCH 23390。MPTP 处理的小鼠似乎对拮抗剂更敏感，即比对照小鼠响应更早且对更低剂量的拮抗剂。用放线菌酮预处理可防止拮抗剂诱导的 AAAD 活性升高。用拮抗剂治疗后，L-3,4-二羟基苯丙氨酸 (L-DOPA) 和 5-磷酸吡哆醛的表观 K(m) 值似乎没有变化。在亚慢性施用多巴胺受体拮抗剂或用利血平治疗后也观察到AAAD活性增加。单剂量的选择性多巴胺受体激动剂对 AAAD 活性没有影响。相反，服用 L-DOPA、喹吡罗或 SKF 23390 7 天会降低纹状体中的 AAAD 活性。我们得出结论，AAAD 在纹状体中通过多巴胺能受体进行调节。
AromatiC L-amino acid decarboxylase (AAAD) activity is enhanced in the striatum of control and MPTP-treated mice after administration of a single dose of the dopamine receptor antagonists haloperidol, sulpiride. and SCH 23390. MPTP-treated mice appear more sensitive to the antagonists, i.e., respond earlier and to lower doses of antagonists than control mice. The rise of AAAD activity induced by the antagonists is prevented by pretreatment with cycloheximide. The apparent K(m) values for L-3,4-dihydroxyphenylalanine (L-DOPA) and pyridoxal 5-phosphate appear unchanged after treatment with the antagonists. Increased AAAD activity was observed also after subchronic administration Of dopamine receptor antagonists or treatment with reserpine. A single dose of a selective dopamine receptor agonists had no effect on AAAD activity. In contrast, administration of L-DOPA, quinpirole, or SKF 23390 for 7 days lowers AAAD activity in the striatum. We conclude that AAAD is modulated in striatum via dopaminergic receptors.