GmCRY1s modulate gibberellin metabolism to regulate soybean shade avoidance in response to reduced blue light
GmCRY1s modulate gibberellin metabolism to regulate soybean shade avoidance in response to reduced blue light
复制标题
GmCRY1s 调节赤霉素代谢以调节大豆对蓝光减少的避荫反应
DOI：
10.1016/j.molp.2020.11.016
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发表时间：
2021-02-01
期刊：
影响因子：
27.5
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Lyu, Xiangguang;Cheng, Qican;Liu, Bin
大豆是一种重要的豆科作物，它表现出典型的遮荫回避综合征(SAS)，包括茎的过度伸长，导致密度耕作条件下倒伏和减产。在此，我们比较了低红光与远红光比(R：FR)和低蓝光(LBL)两种遮荫信号对大豆生长状态的影响，发现LBL主要诱导茎的过度伸长。我们利用CRISPR-Cas9工程突变体研究了大豆中7个隐花色素(GmCRY)的功能，发现4个GmCRY1在介导LBL诱导的SAS过程中重叠。光激活的GmCRY1增加了bZIP转录因子STF1和STF2的丰度，这两个转录因子直接上调了编码GA-2氧化酶的基因的表达，从而使GA-1失活，抑制了茎的伸长。值得注意的是，在密植和间作条件下，GmCRY1b高表达系表现出比野生型对照多方面的农艺优势。我们的研究证实了GmCRY1介导的信号与GA代谢途径在调节LBL诱导的大豆SAS中的整合。这也为今后培育抗倒伏、高产的大豆品种提供了一个很有前途的选择。
Soybean is an important legume crop that displays the classic shade avoidance syndrome (SAS), including exaggerated stem elongation, which leads to lodging and yield reduction under density farming conditions. Here, we compared the effects of two shade signals, low red light to far-red light ratio (R:FR) and low blue light (LBL), on soybean status and revealed that LBL predominantly induces excessive stem elongation. We used CRISPR-Cas9-engineered Gmcry mutants to investigate the functions of seven cryptochromes (GmCRYs) in soybean and found that the four GmCRY1s overlap in mediating LBL-induced SAS. Light-activated GmCRY1s increase the abundance of the bZIP transcription factors STF1 and STF2, which directly upregulate the expression of genes encoding GA2 oxidases to deactivate GA-1 and repress stem elongation. Notably, GmCRY1b overexpression lines displayed multiple agronomic advantages over the wild-type control under both dense planting and intercropping conditions. Our study demonstrates the integration of GmCRY1-mediated signals with the GA metabolic pathway in the regulation of LBL-induced SAS in soybean. It also provides a promising option for breeding lodging-resistant, high-yield soybean cultivars in the future.