A method to differentiate between thyroglobulin derived from normal thyroid tissue and from thyroid carcinoma based on analysis of reactivity to lectins.
A method to differentiate between thyroglobulin derived from normal thyroid tissue and from thyroid carcinoma based on analysis of reactivity to lectins.
复制标题
一种基于凝集素反应性分析来区分源自正常甲状腺组织和甲状腺癌的甲状腺球蛋白的方法。
DOI：
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发表时间：
1998
影响因子：
4.6
通讯作者：
Y. Kasuga
中科院分区：
文献类型：
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作者：
M. Maruyama;R. Kato;S. Kobayashi;Y. Kasuga
目的
甲状腺癌细胞（C-Tg）产生的甲状腺球蛋白（Tg）糖链组成与正常甲状腺组织（N-Tg）不同。本研究基于C-Tg和N-Tg与凝集素反应性的差异，设计了一种检测甲状腺癌Tg的新方法。
材料和方法
将从各种甲状腺组织获得的甲状腺球蛋白制剂与凝集素一起孵育，通过酶联免疫吸附测定法测定上清液中凝集素未结合的Tg（ub-Tg）相对于未用凝集素处理的Tg的量，并表示为ub-Tg（%）。此外，为了进一步研究C-Tg和N-Tg之间糖基化的差异，在Western印迹后在硝酸纤维素膜上分析了用金黄色葡萄球菌V8蛋白酶消化的伴刀豆球蛋白A与Tg的结合。
结果
对于伴刀豆球蛋白A和蓖麻凝集素-120，乳头状癌的C-Tg中的ub-Tg（%）显著高于Graves病、良性甲状腺肿和正常甲状腺组织的Tg。伴刀豆球蛋白A似乎没有结合到Tg从乳头状癌V8治疗后，通过Western印迹分析。伴刀豆球蛋白A治疗后，甲状腺滤泡性腺瘤中的Ub-Tg（%）显著高于滤泡性癌中的C-Tg，而滤泡性癌和正常甲状腺组织中的Ub-Tg（%）无差异。
结论
这些结果表明，我们的新方法可以区分C-Tg和乳头状癌和N-Tg，以及甲状腺组织标本中滤泡状癌和滤泡状腺瘤。因此，这种类型的分析可能适用于区分甲状腺抽吸物中的C-Tg和N-Tg，用于甲状腺癌的连续细胞诊断。
OBJECTIVE
The composition of sugar chains on thyroglobulin (Tg) produced in thyroid carcinoma cells (C-Tg) is different from Tg produced in normal thyroid tissues (N-Tg). In this study, we designed a new method for detecting Tg derived from thyroid carcinoma based on the differences between C-Tg and N-Tg in the reactivity with lectins.
MATERIALS AND METHODS
Thyroglobulin preparations obtained from various thyroid tissues were incubated with lectins, and the amount of lectin-unbound Tg (ub-Tg) in the supernatant relative to Tg untreated with lectin was determined by enzyme-linked immunosorbent assay and expressed as ub-Tg(%). In addition, to study further the differences in glycosylation between C-Tg and N-Tg, concanavalin A binding to Tg digested with Staphylococcus aureus V8 protease was analyzed on nitrocellulose membrane after Western blotting.
RESULTS
The ub-Tg(%) in C-Tg from papillary carcinoma was significantly higher than in Tg from Graves' disease, benign goiter, and normal thyroid tissue for both concanavalin A and ricinus communis agglutinin-120. Concanavalin A did not appear to bind to Tg from papillary carcinoma after V8 treatment by Western blot analysis. The ub-Tg(%) in Tg from follicular adenoma was significantly higher than C-Tg from follicular carcinoma, whereas there were no differences in ub-Tg(%) between follicular carcinoma and normal thyroid tissue in concanavalin A treatment.
CONCLUSIONS
These results suggest our new methods can distinguish both between C-Tg from papillary carcinoma and N-Tg, and between follicular carcinoma and follicular adenoma in thyroid tissue specimens. Thus, this type of analysis may be applicable to differentiate C-Tg from N-Tg in thyroid aspirates for the adjunctive cytodiagnosis of thyroid carcinoma.