Automated fibrinogen determination.
Automated fibrinogen determination.
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自动纤维蛋白原测定。
DOI：
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发表时间：
1971
影响因子：
3.5
通讯作者：
R. Menga
中科院分区：
文献类型：
--
作者：
E. Morse;S. Panek;R. Menga
本文介绍了一种简便、快速、准确的半自动微量法测定血浆纤维蛋白原。将十分之一毫升血浆稀释在1.9毫升中。巴比妥缓冲液（pH 7.5）。加入0.1 ml后，通过电子计时器测定凝血时间。凝血酶（100 U.每毫升）至0.1ml。稀释血浆从校准曲线读取纤维蛋白原水平。通过化学方法确认异常水平。两种方法所得结果之间的差异可能提示纤维蛋白原结构异常或存在纤维蛋白裂解产物。
A simple, rapid, and accurate semiautomated micromethod for the determination of plasma fibrinogen is described. One tenth milliliter of plasma is diluted in 1.9 ml. barbital buffer (pH 7.5). The clotting time is determined by an electronic tinier after addition of 0.1 ml. thrombin (100 U. per ml.) to 0.1 ml. diluted plasma. The fibrinogen level is read from a calibration curve. Abnormal levels are confirmed by a chemical method. Discrepancies between results obtained by the two methods may be a clue to structural abnormalities of fibrinogen or the presence of fibrin split products.