Low molecular weight mitogenic factor produced by BRL-3A cultured rat liver cells.
Low molecular weight mitogenic factor produced by BRL-3A cultured rat liver cells.
复制标题
BRL-3A 培养的大鼠肝细胞产生的低分子量有丝分裂因子。
DOI：
10.1016/0006-291x(81)90704-x
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发表时间：
1981
影响因子：
3.1
通讯作者：
Pang,KJ
中科院分区：
文献类型：
--
作者：
Straus,DS;Coppock,DL;Pang,KJ
从BRL-3A大鼠肝细胞培养基中分离到一种新的有丝分裂因子。在Dowex 50上进行离子交换层析，然后在1 M乙酸中在Sephadex G-75上进行凝胶过滤层析，通过两步程序对该因子进行了部分纯化。该因子在Sephadex G-75柱的低分子量区洗脱，位于增殖刺激活性的三个峰后面。该因子通过胰蛋白酶和二硫苏糖醇灭活，表明它是一种含有二硫键的肽。与增殖刺激活性不同，新因子在静止的鸡成纤维细胞中仅微弱地刺激DNA合成，而在静止的NIL8仓鼠细胞、BALB c 3T3细胞和IMR-90人成纤维细胞中则强烈地刺激DNA合成。
A new mitogenic factor has been isolated from medium conditioned by BRL-3A rat liver cells. The factor has been partially purified by a two step procedure involving ion exchange chromatography on Dowex 50 followed by gel filtration chromatography on Sephadex G-75 in 1 M acetic acid. The factor is eluted from the Sephadex G-75 column in the low molecular weight region, behin three peaks of multiplication stimulating activity. The factor is inactivated by treatment with trypsin and dithiothreitol, suggesting that it is a peptide that contains a disulfide linkage. Unlike multiplication stimulating activity, the new factor only weakly stimulates DNA synthesis in quiescent chick fibroblasts, whereas it strongly stimulates DNA synthesis in quiescent NIL8 hamster cells, BALB c 3T3 cells, and IMR-90 human fibroblasts.