Feeding dsSerpin1 Enhanced Metarhizium anisopliae Insecticidal Efficiency against Locusta migratoria manilensis
Feeding dsSerpin1 Enhanced Metarhizium anisopliae Insecticidal Efficiency against Locusta migratoria manilensis
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DOI：
10.3390/agriculture12040538
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发表时间：
2022-04
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影响因子：
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通讯作者：
Junya Wang;Hongmei Li;Yumeng Cheng;Guangjun Wang;X. Nong;B. Luke;Undarmaa Davaasambuu;Guocai Zhang
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文献类型：
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作者：
Junya Wang;Hongmei Li;Yumeng Cheng;Guangjun Wang;X. Nong;B. Luke;Undarmaa Davaasambuu;Guocai Zhang
RNA干扰（RNAi）诱导基因沉默以参与免疫相关途径。以往的研究表明，LmSerpin 1基因上调东亚飞蝗的免疫应答，并且通过注射干扰dsSerpin 1可以增强蝗虫对金龟子绿僵菌的感染。本研究利用丝氨酸蛋白酶抑制剂1基因合成双链RNA，并将其喂给三龄棉铃虫。M. 5 μg、10 μg和20 μg.投喂dsSerpin 1后，L. M. 20 μ gdsSerpin 1的致死率最高，10 μ gdsSerpin 1次之，5 μ gdsSerpin 1的致死率最低。sSerpin 1沉默和dsSerpin 1诱导温度热对M.金龟子，表现出剂量依赖性反应。当L. M.用含20 μg dsRNA和M.绿僵菌（2.5 × 108孢子/g麦麸），3天死亡率显著增加。dsSerpin 1 + M.绿僵菌处理导致60%的6天死亡率，加速了昆虫死亡。这些结果表明，饲喂dsSerpin 1可以有效地控制害虫，并且在前7天内控制效果和dsRNA浓度具有剂量依赖性。其中20 μg是提高杀虫效果的有效剂量。绿僵菌
RNA interference (RNAi) induces gene silencing in order to participate in immune-related pathways. Previous studies have shown that the LmSerpin1 gene upregulates the immune responses of Locusta migratoria manilensis, and that the infection of locusts with Metarhizium anisopliae can be enhanced through the injection of the interfering dsSerpin1. In this study, dsRNA was synthesized from the Serpin1 gene and fed to the third instars of L. m. manilensis at concentrations of 5 μg, 10 μg, and 20 μg. Feeding dsSerpin1 increased the mortality of L. m. manilensis, and 20 μg dsSerpin1 had the highest lethality, followed by 10 μg dsSerpin1, with the lowest being observed at 5 μg dsSerpin1. Serpin1 silencing and temperature fever induction by feeding with dsSerpin1 had a strong synergistic effect on M. anisopliae, showing a dose-dependent response. When L. m. manilensis were fed on a diet containing 20 μg dsRNA and M. anisopliae (2.5 × 108 spores/g wheat bran), 3-day mortalities significantly increased. The dsSerpin1 plus M. anisopliae treatments resulted in a 6-day mortality of 60%, accelerating insect death. These results indicated that feeding with dsSerpin1 could be an effective way to control pests and that the control effect and dsRNA concentration were dose-dependent within the first seven days. In particular, 20 μg was an effective dosage to enhance the insecticidal efficiency of M. anisopliae.