The U2AF35-related protein Urp contacts the 3′ splice site to promote U12-type intron splicing and the second step of U2-type intron splicing
The U2AF35-related protein Urp contacts the 3′ splice site to promote U12-type intron splicing and the second step of U2-type intron splicing
复制标题
DOI：
10.1101/gad.1974810
复制
发表时间：
2010-11-01
影响因子：
10.5
通讯作者：
Green, Michael R.
中科院分区：
文献类型：
--
作者：
Shen, Haihong;Zheng, Xuexiu;Green, Michael R.
U2AF35相关蛋白Urp先前已涉及主要类别的U2型内含子的剪接。在这里，我们表明，Urp也需要剪接的小类U12型内含子。Urp以ATP依赖的方式被募集到U12型内含子39剪接位点，在那里它促进剪接体复合物的形成。值得注意的是，Urp也接触U2型内含子的39剪接位点，但在这种情况下，是剪接的第二步所特别需要的。因此，通过识别共同的剪接元件，Urp促进了U2型和U12型内含子剪接的不同步骤。
The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 39 splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 39 splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.