Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
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DOI：
10.1016/j.jviromet.2016.05.018
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发表时间：
2016-09-01
影响因子：
3.1
通讯作者：
Boonham, Neil
中科院分区：
文献类型：
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作者：
Rozado-Aguirre, Zurine;Adams, Ian;Boonham, Neil
一种新的托拉多病毒（暂定名为胡萝卜托拉多病毒（CaTV））是在调查胡萝卜内部血管坏死的下一代测序研究后偶然发现的。最接近的病毒是 2011 年在荷兰发现的生菜坏死卷叶病毒 (LNLCV) 和 2014 年在韩国发现的益母草黄斑驳病毒 (MYMoV)。设计了逆转录酶 PCR (RT-PCR) 和 RT-qPCR 的引物，目的是测试从田间收集的植物样本中是否存在病毒。这两种方法都成功地从受感染的样本中扩增了目标，但没有从健康的对照样本中扩增出目标。还针对其他已知的胡萝卜病毒检查了 CaTV 检测的特异性，未发现交叉反应。方法之间的比较研究表明 RT-qPCR 是最可靠的方法，在 RT-PCR 失败的样本中给出阳性结果。 RT-qPCR 后的 Ct 值评估和直接比较表明这是由于灵敏度提高。之前发布的 Torradovirus 属特异性 RT-PCR 引物经过测试并显示可检测 CaTV。此外，进行的病毒传播实验表明，与同属的其他物种不同，胡萝卜托拉多病毒可以通过蚜虫传播。 (C) 2016 Elsevier B.V. 保留所有权利。
A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Motherwort yellow mottle virus (MYMoV) found in Korea in 2014. Primers for reverse transcriptase-PCR (RT-PCR) and RT-qPCR were designed with the aim of testing for the presence of virus in plant samples collected from the field. Both methods successfully amplified the target from infected samples but not from healthy control samples. The specificity of the CaTV assay was also checked against other known carrot viruses and no cross-reaction was seen. A comparative study between methods showed RT-qPCR was the most reliable method, giving positive results in samples where RT-PCR fails. Evaluation of the Ct values following RT-qPCR and a direct comparison demonstrated this was due to improved sensitivity. The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV. Also, virus transmission experiments carried out suggest that unlike other species of the same genus, Carrot torrado virus could be aphid-transmitted. (C) 2016 Elsevier B.V. All rights reserved.