Axo-glial interactions regulate the localization of axonal paranodal proteins.
Axo-glial interactions regulate the localization of axonal paranodal proteins.
复制标题
轴突相互作用调节轴突偏阳蛋白的定位。
DOI：
10.1083/jcb.147.6.1145
复制
发表时间：
1999-12-13
影响因子：
7.8
通讯作者：
Popko, B
中科院分区：
文献类型：
--
作者：
Dupree, J L;Girault, J A;Popko, B
不能合成大量半乳糖脂的髓鞘的小鼠在中枢和外周神经系统中表现出破坏的结旁轴-神经胶质相互作用。使用这些突变体，我们分析了轴-神经胶质细胞的相互作用中发挥的作用，在该地区的节点的朗维尔轴突蛋白分布的建立。而结蛋白质，钠通道，ankeplatin G，和神经成束蛋白的集群只有轻微的影响，钾通道和paranodin，蛋白质，通常集中在该地区并列的节点的分布，显着改变。钾离子通道通常集中在旁阳极/腹主动脉，但不限于此区域，而是在半乳糖脂缺乏小鼠的整个节间检测到。Paranodin/contactin-associated protein（Caspr）是一种潜在的轴突-髓鞘结合的神经元介导物，其不集中于结旁区域，而是沿沿着结间区域弥散分布。总的来说，这些研究结果表明，髓鞘半乳糖脂是必不可少的正确形成的轴-胶质细胞的相互作用，并证明，在这些相互作用的破坏结果在结旁轴膜的分子组织的深刻异常。
Mice incapable of synthesizing the abundant galactolipids of myelin exhibit disrupted paranodal axo-glial interactions in the central and peripheral nervous systems. Using these mutants, we have analyzed the role that axo-glial interactions play in the establishment of axonal protein distribution in the region of the node of Ranvier. Whereas the clustering of the nodal proteins, sodium channels, ankyrinG, and neurofascin was only slightly affected, the distribution of potassium channels and paranodin, proteins that are normally concentrated in the regions juxtaposed to the node, was dramatically altered. The potassium channels, which are normally concentrated in the paranode/juxtaparanode, were not restricted to this region but were detected throughout the internode in the galactolipid-defi- cient mice. Paranodin/contactin-associated protein (Caspr), a paranodal protein that is a potential neuronal mediator of axon-myelin binding, was not concentrated in the paranodal regions but was diffusely distributed along the internodal regions. Collectively, these findings suggest that the myelin galactolipids are essential for the proper formation of axo-glial interactions and demonstrate that a disruption in these interactions results in profound abnormalities in the molecular organization of the paranodal axolemma.