Comparative proteomics of related symbiotic mussel species reveals high variability of host–symbiont interactions
Comparative proteomics of related symbiotic mussel species reveals high variability of host–symbiont interactions
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DOI：
10.1038/s41396-019-0517-6
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发表时间：
2019-11
期刊：
影响因子：
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通讯作者：
R. Ponnudurai;Stefan E. Heiden;L. Sayavedra;Tjorven Hinzke;M. Kleiner;Christian Hentschker;H. Felbeck
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文献类型：
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作者：
R. Ponnudurai;Stefan E. Heiden;L. Sayavedra;Tjorven Hinzke;M. Kleiner;Christian Hentschker;H. Felbeck
深海Bathymodiolusmussels和它们的化能自养共生体是互惠宿主微生物协会的代表。然而，宿主-共生体相互作用如何在相关宿主和共生体物种之间在分子水平上变化仍然不清楚。因此，我们比较了PacificB的宿主和共生体元蛋白组。thermophilus，托管一个硫营养共生体，和approximaticB. azoricus，含有两种共生体，一种硫营养菌和一种甲烷营养菌。我们确定了宿主和共生体之间的代谢支持的共同策略，例如宿主对硫化物的氧化，这为硫营养共生体提供了硫代硫酸盐库，以及可以为宿主提供共生体衍生氨基酸的循环机制。然而，这些过程的表达水平在两种共生体之间有很大差异。在基因组比较的支持下，我们的结果进一步揭示了B.嗜热共生体这些发现意味着宿主与微生物的相互作用可能存在很大差异，即使是在密切相关的系统之间也是如此。
Deep-seaBathymodiolusmussels and their chemoautotrophic symbionts are well-studied representatives of mutualistic host–microbe associations. However, how host–symbiont interactions vary on the molecular level between related host and symbiont species remains unclear. Therefore, we compared the host and symbiont metaproteomes of PacificB. thermophilus, hosting a thiotrophic symbiont, and AtlanticB. azoricus, containing two symbionts, a thiotroph and a methanotroph. We identified common strategies of metabolic support between hosts and symbionts, such as the oxidation of sulfide by the host, which provides a thiosulfate reservoir for the thiotrophic symbionts, and a cycling mechanism that could supply the host with symbiont-derived amino acids. However, expression levels of these processes differed substantially between both symbioses. Backed up by genomic comparisons, our results furthermore revealed an exceptionally large repertoire of attachment-related proteins in theB. thermophilussymbiont. These findings imply that host–microbe interactions can be quite variable, even between closely related systems.