EFFECTS OF LOCAL APPLICATION OF 5-HT AND 8-OH-DPAT INTO THE DORSAL AND MEDIAN RAPHE NUCLEI ON CORE TEMPERATURE IN THE RAT
EFFECTS OF LOCAL APPLICATION OF 5-HT AND 8-OH-DPAT INTO THE DORSAL AND MEDIAN RAPHE NUCLEI ON CORE TEMPERATURE IN THE RAT
复制标题
DOI：
10.1007/bf02244281
复制
发表时间：
1991-01-01
影响因子：
3.4
通讯作者：
HILLEGAART, V
中科院分区：
文献类型：
--
作者：
HILLEGAART, V
用5-羟色胺（10 μ g）或8-OH-DPAT （5 μ g）局部注射于雄性Wistar大鼠背核（DR）或中段核（MR）后，测量其核心温度。注射程序开始后20和60分钟，通过直肠热敏电阻探头测量核心温度。注射量0.5 mu-l，注射针头31G，注射速率0.33 mu-l min-1。为了避免穿透脑导水管，或避免以MR为目标注射中缝核，以30度接近中缝核。将5-HT或5-HT1A激动剂8-OH-DPAT应用于DR可显著降低核心温度，而向MR注射则没有效果。这些结果表明DR在大鼠体温调节中起着重要作用。5-HT1A激动剂8-OH-DPAT导致核心温度降低的事实，以及5-HT1拮抗剂（-）pindolol对5-HT的拮抗以及8-OH-DPAT诱导的降低的观察，表明DR 5-HT1A受体参与大鼠体温调节。
The core temperature of male Wistar rats was measured after local application of 5-HT (10-mu-g) or 8-OH-DPAT (5-mu-g) into the dorsal (DR) or the median raphe (MR) nuclei. The core temperature was measured by a rectal thermistor probe, 20 and 60 min after the injection procedure started. The injected volume was 0.5-mu-l and injections were made by means of 31G needles, at a rate of 0.33-mu-l min-1. The raphe nuclei were approached at 30-degrees in order to avoid penetration of the cerebral aqueduct or to avoid the DR with injections aimed for the MR. The application of 5-HT or the 5-HT1A agonist 8-OH-DPAT into the DR produced a marked decrease in core temperature, whereas injections into the MR had no effect. These results demonstrate an important role for the DR in temperature regulation in the rat. The fact that the 5-HT1A agonist 8-OH-DPAT produced a decrease in core temperature, together with the observation that administration of the 5-HT1 antagonist (-)pindolol antagonized the 5-HT as well as the 8-OH-DPAT-induced decrease, indicates the involvement of DR 5-HT1A receptors in rat thermoregulation.